PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

jimmy88cn

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2 t7 F  F; x: `这是2010出版的 Methods in Molecular Biology 系列 vol.6213 e$ |& L' r4 U$ j3 c- a2 m' {6 [
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以下为该书前言：
' T0 p- t3 F+ @# O' Y8 E  T$ A& B! |Although embryonic stem cells currently enjoy the public limelight and show great promise
0 a; m- `, ^8 Jfor cell based medical therapies, it is the adult stem cells which are responsible for the
/ Z! W( H9 M6 X  C* |2 Qbody’s natural ability to fight disease, heal and recover, or fail and succumb to various9 r& t9 \5 `+ |" F. f) b1 B2 C1 X: ?
maladies. The study of mammalian adult stem cells has surged recently, most likely from a6 B. Q2 Z8 k/ V: X2 o
maturation of stem cell studies in the classical developmental model organisms and in
  q. }/ c9 ]( t% b" P% w: Vhematopoeisis. All the tissues of the body examined so far are generated and regenerated7 M+ ]- a7 ~) o) R) K2 ?: x7 i
from stem cells, it has been an important first step to adapt or devise new methods to
) v/ e- b7 t+ k( u% K7 _  F4 Tidentify and obtain these cells in quantity and purity for further study. Culture techniques& n1 H& P+ Q/ N7 |& T1 _
have been optimized for managing the growth and differentiation of stem cells in vitro; as# E- v' p+ G% T$ {
some stem cells are pluripotent, often the method is to guide the fate of such cells among
! q1 R. H8 W* i7 ~the possible differentiation fates. Much of this work, and that in the classical model organisms,/ {2 b: c0 [1 o! F, r" Z
has helped define the aspects of the stem cell environment or niche that are crucial
4 K; j. i7 q# Z! |' y6 D6 b$ s8 O& ofor both growth and differentiation, and these studies have moved in vivo at increasingly
  k; Z( c6 y9 D5 c( f6 w# Y# }- [higher resolution. Importantly, the in vivo niche is a current target for bioengineering the
1 s6 l, \1 U# n/ y$ lmatrix and signaling factors.
; Q  B) R1 N5 K2 O: i3 F5 l: \Herein, we present methods for studying six types of mammalian stem cells, mammary,0 }5 E# W/ H! C7 h5 ^
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches+ U* @; F8 b$ H3 K
described in these chapters will assist you in obtaining, characterizing, and studying these
2 t# m" R0 e" a7 I" w3 }cells or for adaptation to your favorite stem cells. One final note, should you find the
- i/ N5 r" c9 Uinformation herein valuable and it helps you to make great discoveries in the understanding
7 S( g) h6 K# N" H; o; t& m- I, aof stem cell biology or application, by all means please let us, the authors, know! The1 c9 D- c# w3 g4 K4 U' m' C; y
best way is of course to publish your findings, but falling short of that, please feel free to
3 x8 Y; O# A" t2 V9 zask questions or clarifications. Especially for this reason, our contact information is provided
* O0 r( G; X3 o9 V) J7 A: s3 E" pat the end of this book.
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tspaulzhao

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