PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

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这是2010出版的 Methods in Molecular Biology 系列 vol.6218 ?. i& x) H4 V5 e
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以下为该书前言：
, ~0 z9 r3 i3 Z! y: UAlthough embryonic stem cells currently enjoy the public limelight and show great promise! u: ~& l8 [& A1 R8 `
for cell based medical therapies, it is the adult stem cells which are responsible for the" S. u  t, e: [4 t
body’s natural ability to fight disease, heal and recover, or fail and succumb to various: J1 o3 A. [* Y  _& d
maladies. The study of mammalian adult stem cells has surged recently, most likely from a. A0 ]5 d2 g, P( L# P- j( ]0 |% x
maturation of stem cell studies in the classical developmental model organisms and in: Y- c$ ?- ^6 U9 w
hematopoeisis. All the tissues of the body examined so far are generated and regenerated
5 F- T9 v: v3 D" X/ l; Hfrom stem cells, it has been an important first step to adapt or devise new methods to  m$ x1 \0 x9 W, z. }& U8 P
identify and obtain these cells in quantity and purity for further study. Culture techniques
; ?: ], G4 K% n- k& ]- dhave been optimized for managing the growth and differentiation of stem cells in vitro; as  t* o! I5 ?) V
some stem cells are pluripotent, often the method is to guide the fate of such cells among
0 h3 n/ C; l9 a! i4 p6 ?" Sthe possible differentiation fates. Much of this work, and that in the classical model organisms,
4 q2 O0 ]4 J  o8 `8 Z" Y, Ihas helped define the aspects of the stem cell environment or niche that are crucial4 e7 `  }! q( V" J
for both growth and differentiation, and these studies have moved in vivo at increasingly
- b- K, _8 _. Yhigher resolution. Importantly, the in vivo niche is a current target for bioengineering the
% C; g- W, i! ]7 A/ wmatrix and signaling factors.1 ~- w0 U0 L* E2 T( m1 A
Herein, we present methods for studying six types of mammalian stem cells, mammary,; Y" J: Q5 Q( Y7 ~6 K3 r- n
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches1 B7 s* e* \6 ?% B) ~: Q0 V
described in these chapters will assist you in obtaining, characterizing, and studying these
; Q: o. h- v! O3 Pcells or for adaptation to your favorite stem cells. One final note, should you find the
3 s, p% i# b" R0 ?2 G& Winformation herein valuable and it helps you to make great discoveries in the understanding/ ?) t4 T  n7 j; S" u8 n
of stem cell biology or application, by all means please let us, the authors, know! The) h) f# c2 Z* u; }4 V6 r, n
best way is of course to publish your findings, but falling short of that, please feel free to6 w) T, E- J" ~. J
ask questions or clarifications. Especially for this reason, our contact information is provided
% v# C* `( Z, I1 R% B$ }8 U- M+ W: R( Fat the end of this book.# d* F/ R& s! l" H0 {4 y

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