询问一个关于基质胶的问题！

shihuijunm

Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. * c6 n6 {: L2 A
  Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells / c/ B# J( {2 h6 m/ n' Y' x9 i2 N

4 d# l- h9 Q' j. P/ D& ]Caution：ECM gel may be stored up to 72 hours at 2-8 °C.
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8 s/ V% c5 e% K( h' vOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.* ]. R! _* C$ ^8 i. i  P+ _
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Properties- k2 }( g! E$ _1 o2 W2 {
sterility dialyzed against chloroform 7 q3 f' G. g5 e4 m1 U* A
form liquid 9 e& p) B0 C* w* N* }
concentration 8 - 12 mg/mL 4 A( O: ^" W# d$ n, V
surface coverage 6‑10 μg/cm2 # O( a" E. f2 a& j& o
total impurities endotoxin, tested
' w; w- b, a- i4 Bsuitability cell culture tested $ \+ v9 S* r9 q, o2 L) R) b
shipped in dry ice ( _) u( n" X6 O" i; n' ]$ w- K
storage temp. −20°C

ahfjwangrui

回复 2# 胡问成
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    我是看很多文献说是在培养干细胞时铺板用

胡问成

回复 1# ahfjwangrui
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, \7 V9 ?: G. U0 d. q- V/ E( G7 F    看你要用肿瘤干细胞做什么，基质胶一般用来检测细胞的侵袭、生长能力，如果你要做肿瘤干细胞的克隆、生长能力可以用软琼脂糖克隆实验，不过比较麻烦些