求用小鼠胚胎做western的方法~~

hongjie88

RT~~
. O, Y# `. z3 `( a' d$ Y要用多少胚胎？怎么裂解？。。。& L& p) J3 Y0 V" n- Z
最好是有做过的高手传授一下~~

lfl-01

同问~

是鸟就要去飞

有意思，我也来看看

iseeyou1210

lz要看多大的胚胎？我觉得在7天以后一个胚胎就够至少2个wb了，要是7天以前的，可能要pool好几个，我做过13天fetal liver的，直接取liver然后用匀浆器操作就可以，用一般的ripa buffer，没有问题。

hongjie88

回复 4# iseeyou1210 6 n' d) I0 ^4 o4 A' _8 g# z8 g

' a4 j( t  ]0 K多谢了

hongjie88

如果是8-cell----blastocyst的胚胎得用多少？

iseeyou1210

i think it is better to do RT rather than do the blastocyst wb.& |# {% X+ w; f& C
Think about you need maybe 10,000 cells to wb and then you need 10,000/8= around 1000 blastocysts to do the protein extraction.. E0 z/ F/ ?8 }8 x9 @
I think no way you can have some results.