求用小鼠胚胎做western的方法~~

hongjie88

RT~~+ r5 [9 O5 B! y: o
要用多少胚胎？怎么裂解？。。。0 J5 ^( B+ N4 m. g
最好是有做过的高手传授一下~~

lfl-01

同问~

是鸟就要去飞

有意思，我也来看看

iseeyou1210

lz要看多大的胚胎？我觉得在7天以后一个胚胎就够至少2个wb了，要是7天以前的，可能要pool好几个，我做过13天fetal liver的，直接取liver然后用匀浆器操作就可以，用一般的ripa buffer，没有问题。

hongjie88

回复 4# iseeyou1210 . U6 q* q) _0 K/ w9 x0 o" y! N1 f

, X+ A1 J$ H" k+ x4 A( R. k多谢了

hongjie88

如果是8-cell----blastocyst的胚胎得用多少？

iseeyou1210

i think it is better to do RT rather than do the blastocyst wb.
% [0 B1 L. S3 M" M6 y7 nThink about you need maybe 10,000 cells to wb and then you need 10,000/8= around 1000 blastocysts to do the protein extraction.* K& J# [* S; r$ ~9 g
I think no way you can have some results.