求用小鼠胚胎做western的方法~~

hongjie88

RT~~
5 U- |9 k' ]7 r' [要用多少胚胎？怎么裂解？。。。* ?# i4 ]- n" C1 a  o5 ]. `# `
最好是有做过的高手传授一下~~

lfl-01

同问~

是鸟就要去飞

有意思，我也来看看

iseeyou1210

lz要看多大的胚胎？我觉得在7天以后一个胚胎就够至少2个wb了，要是7天以前的，可能要pool好几个，我做过13天fetal liver的，直接取liver然后用匀浆器操作就可以，用一般的ripa buffer，没有问题。

hongjie88

回复 4# iseeyou1210
& L( `6 k' N7 h) ~8 {$ c" ~+ [
* n6 I% R! \; c# ?( D: E多谢了

hongjie88

如果是8-cell----blastocyst的胚胎得用多少？

iseeyou1210

i think it is better to do RT rather than do the blastocyst wb.
  \! |8 z. Y8 |% T( F$ C: t  ]Think about you need maybe 10,000 cells to wb and then you need 10,000/8= around 1000 blastocysts to do the protein extraction.
4 v% a: v# ~5 X9 F0 vI think no way you can have some results.