求用小鼠胚胎做western的方法~~

hongjie88

RT~~
5 o! b/ @" _3 P要用多少胚胎？怎么裂解？。。。& T6 |" w; j" c$ y2 M. l
最好是有做过的高手传授一下~~

lfl-01

同问~

是鸟就要去飞

有意思，我也来看看

iseeyou1210

lz要看多大的胚胎？我觉得在7天以后一个胚胎就够至少2个wb了，要是7天以前的，可能要pool好几个，我做过13天fetal liver的，直接取liver然后用匀浆器操作就可以，用一般的ripa buffer，没有问题。

hongjie88

回复 4# iseeyou1210 1 I1 x- Z/ ]. c7 V

0 I* x: G2 b& @5 C; X多谢了

hongjie88

如果是8-cell----blastocyst的胚胎得用多少？

iseeyou1210

i think it is better to do RT rather than do the blastocyst wb.1 o4 g: p6 d$ z# _! x+ [; Q0 `- p
Think about you need maybe 10,000 cells to wb and then you need 10,000/8= around 1000 blastocysts to do the protein extraction.0 R+ n" o: S8 P  b; z. H( Y! c
I think no way you can have some results.