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本帖最后由 细胞海洋 于 2011-4-27 16:16 编辑 0 J- l* B% y! E: l
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杂志：Cytotechnology (2011) 63:67–80
- k C; B- u' j题目 ：Long-term cultures of stem/progenitor cells from lobular and ductal breast carcinomas under non-adherent conditions- I7 S. k" q# V; n6 y
作者：Agostina Nardone • Sara Corvigno •% [0 U6 t1 W M7 l& q3 M9 l+ ^
Annalisa Brescia • Daniel D’Andrea •
1 @+ G0 G$ |3 b+ U& }. q- V1 y Gennaro Limite • Bianca Maria Veneziani
9 l% l+ Q, e8 P, G" Z2 N. i& g文中提到 “To test the ability of LoBS and DuBS cells to form mammospheres, cells were dissociated and seeded, by serial dilution, in ultra low attachment surface 24-well plates in DMEM/F12 plus 0.5% FCS medium (low serum). The final cell dilutions ranged from 10 to 1,000 cells/well. Sphere formation experiments were carried out at least four times per sample.”7 e4 }; U) q. P/ \1 Y
结果如图
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, [( u) S# J m% R% q- ?, D最近养克隆球采用无血清培养基加EGF.FGF.可怎么也不成功。看到这篇文献后有点心动，也想试一试。但血清又有促进细胞分化和贴壁的作用，低血清养出的克隆球和无血清培养出的有可比性吗？
; h$ ]- o" t/ R, v9 i) }0 `! u希望各位可以发表一下自己的意见！" u% f* D' J5 j- u$ g3 a; K( T
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发表于 2011-3-7 10:12
发表于 2011-3-7 13:56
发表于 2011-3-7 19:59
