小鼠胚胎干细胞定向分化为生殖细胞（精子或卵子）的进展和方法？

mubaiyan

小鼠胚胎干细胞定向分化为生殖细胞，有人在做没？这个难度系数怎么样啊，我是新手，正要接触这一块，大家有什么好的建议可以给我啊？谢谢了
$ u, l3 s/ c* P  W9 B1 S/ F国内外做的怎么样，这个分化诱导方案确定了吗？

yangpan521

回复 hcluo 的帖子: E; W, P# X  s4 U2 \
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给我一些干细胞向生殖细胞分化的文献吧 谢谢了

hcluo

这个文献挺多的啊。我这里有一些关于这个方面的，大家可以交流一下。: R* L6 T( w9 ~6 b2 s5 l
Derivation of Oocytes from Mouse Embryonic Stem Cells. Science 300 (5623), 1251-1256 (2003).
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: b+ e& _4 p# D" R/ CDerivation of embryonic germ cells and male gametes from embryonic stem cells. Nature 427 (6970), 148-154 (2004).9 x, @. ]8 U9 e& t1 \% p6 j
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Mouse Embryonic Stem Cells Form Follicle-Like Ovarian Structures but Do Not Progress Through Meiosis. Stem Cells 24 (8), 1931-1936 (2006).& b4 M, W8 S+ p) @" m

: Q' `% i: P0 G5 m In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes. Cloning and Stem Cells 9 (4), 535-548 (2007).
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. t3 P6 a; W# a( M4 M5 T' ^ Induction of oocyte-like cells from mouse embryonic stem cells by co-culture with ovarian granulosa cells. Differentiation 75 (10), 902-911 (2007).
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In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice. Developmental Cell 11 (1), 125-132 (2006).

ndwzf

做这个有什莫意义呢？换个题吧。死胡同

fwyou

很难 系数高啊