小鼠胚胎干细胞定向分化为生殖细胞（精子或卵子）的进展和方法？

mubaiyan

小鼠胚胎干细胞定向分化为生殖细胞，有人在做没？这个难度系数怎么样啊，我是新手，正要接触这一块，大家有什么好的建议可以给我啊？谢谢了
+ X4 P7 T; ~5 y. P3 o  M1 T& N8 W国内外做的怎么样，这个分化诱导方案确定了吗？

fwyou

很难 系数高啊

ndwzf

做这个有什莫意义呢？换个题吧。死胡同

hcluo

这个文献挺多的啊。我这里有一些关于这个方面的，大家可以交流一下。! r" N1 ]( C1 B# S
Derivation of Oocytes from Mouse Embryonic Stem Cells. Science 300 (5623), 1251-1256 (2003).
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. g9 e* k2 n, E" e+ zDerivation of embryonic germ cells and male gametes from embryonic stem cells. Nature 427 (6970), 148-154 (2004).) E0 u& Y  s6 a& V* p4 e( [
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Mouse Embryonic Stem Cells Form Follicle-Like Ovarian Structures but Do Not Progress Through Meiosis. Stem Cells 24 (8), 1931-1936 (2006).! m& ~4 o" s" t. D8 w* Z9 ?
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In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes. Cloning and Stem Cells 9 (4), 535-548 (2007).7 f% S* o0 j, v- r7 Q- H' a( f; ?

6 B: W! m+ L9 m Induction of oocyte-like cells from mouse embryonic stem cells by co-culture with ovarian granulosa cells. Differentiation 75 (10), 902-911 (2007).# z* S" r% v' A' G
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In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice. Developmental Cell 11 (1), 125-132 (2006).

yangpan521

回复 hcluo 的帖子/ w6 D+ [) _' ^" b9 e
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给我一些干细胞向生殖细胞分化的文献吧 谢谢了