小鼠胚胎干细胞定向分化为生殖细胞（精子或卵子）的进展和方法？

mubaiyan

小鼠胚胎干细胞定向分化为生殖细胞，有人在做没？这个难度系数怎么样啊，我是新手，正要接触这一块，大家有什么好的建议可以给我啊？谢谢了
3 L4 h% h; N; {2 u1 \$ k国内外做的怎么样，这个分化诱导方案确定了吗？

fwyou

很难 系数高啊

ndwzf

做这个有什莫意义呢？换个题吧。死胡同

hcluo

这个文献挺多的啊。我这里有一些关于这个方面的，大家可以交流一下。
. T- G0 \% k9 ~- H+ i9 ]Derivation of Oocytes from Mouse Embryonic Stem Cells. Science 300 (5623), 1251-1256 (2003).: \4 N2 z3 `3 ^9 y" o! \2 l
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Derivation of embryonic germ cells and male gametes from embryonic stem cells. Nature 427 (6970), 148-154 (2004).: F; n5 x; t! z/ p( s
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Mouse Embryonic Stem Cells Form Follicle-Like Ovarian Structures but Do Not Progress Through Meiosis. Stem Cells 24 (8), 1931-1936 (2006).: a8 A9 d9 `* q% N: Z( n

& g7 g7 i+ ^! T  @7 `( Q In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes. Cloning and Stem Cells 9 (4), 535-548 (2007).* g+ T4 I! |* V

9 o! n5 D7 x1 v3 j+ B: z6 n6 c! b Induction of oocyte-like cells from mouse embryonic stem cells by co-culture with ovarian granulosa cells. Differentiation 75 (10), 902-911 (2007).
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8 [1 b2 }* t2 pIn Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice. Developmental Cell 11 (1), 125-132 (2006).

yangpan521

回复 hcluo 的帖子- s6 L; u9 D) s4 O- v7 l+ N% W/ R* f1 F3 z
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给我一些干细胞向生殖细胞分化的文献吧 谢谢了