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主题：无DMSO脐带血间充质干细胞的冻存) d1 q$ b1 j/ ]& e3 l1 t* C% ]5 c! F
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说明：原文来源自http://www.ncbi.nlm.nih.gov/pubmed/21468457，由干细胞之家新闻小组成员deron翻译（转帖请注明）。, o/ X, F! l& g0 J, D2 f
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翻译内容：/ S4 H- \: {6 k+ X
脐带血间充质干细胞（UCB来源MSCs）的冻存是其细胞移植治疗临床应用中关键的一步。在MSCs的冻存中，DMSO已经广泛用做冷冻保护剂（CPA）。然而，众所周之DMSO对人体有毒副作用。本研究致力于发展无DMSO的CPA溶液用于脐带血间充质干细胞的冻存，其成分用乙二醇（EG）、1,2-丙二醇（PG）和蔗糖为基本CPAs，辅以聚乙烯醇（PVA）作添加物。脐带血间充质干细胞分别通过投入液氮中做玻璃化冻存以及通过可视化DSC新设备做程序降温冷冻冻存。解冻的脐带血间充质干细胞通过台盼蓝排除检测法测定活力。结果显示脐带血间充质干细胞玻璃化冻存中，解冻活力在加入PVA后从71.2%升至95.4%；但是其程控降温冻存的活力，仅仅从不到10% 升到45%。这些结果显示PVA在脐带血间充质干细胞的冻存中起到正相关作用，同时说明添加PVA的无DMSO的CPA溶液的玻璃化冻存可成为脐带血间充质干细胞冻存的一个有效方案。& H9 m( D8 j! v7 D& a4 v8 V
原摘要：Cryopreservation of umbilical cord blood-derived mesenchymal stem cells (UCB-derived MSCs) is crucial step for its clinical applications in cell transplantation therapy. In the cryopreservation of MSCs, dimethyl sulfoxide has been widely used as a cryoprotectant (CPA). However, it has been proved that DMSO has toxic side effects to human body. In this study, DMSO-free CPA solutions which contained ethylene glycol (EG), 1, 2-propylene glycol (PG) and sucrose as basic CPAs, supplemented with polyvinyl alcohol (PVA) as an additive, were developed for the cryopreservation of UCB-derived MSCs. The cryopreservation of UCB-derived MSCs was achieved by vitrification via plunging into liquid nitrogen and by programmed freezing via an optical-DSC system respectively. The viability of thawed UCB-derived MSCs was tested by trypan blue exclusion assay. Results showed that the viability of thawed UCB-derived MSCs was enhanced from 71.2% to 95.4% in the presence of PVA for vitrification, but only < 10% to 45% of viability was found for programmed freezing. These results indicate that PVA exerts a beneficial effect on the cryopreservation of UCB-derived MSCs and suggest the vitrification in combination with the dimethyl sulfoxide free CPA solutions supplemented with PVA would be an efficient protocol for the cryopreservation of UCB-derived MSCs
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原文链接：http://www.ncbi.nlm.nih.gov/pubmed/21468457（求原文） |
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发表于 2011-4-20 17:17
发表于 2011-4-21 14:41
