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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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( E' e( x0 S" `+ \; `" kTable of Contents" H0 @7 d) d" o4 H# b0 _/ m* r
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning/ m j6 b% n8 n; ?2 I
Chapter 2: Bacteriophage and Its Vectors0 i' R9 G: A2 g( \0 o. _
Chapter 3: Working with Bacteriophage M13 Vectors; ~7 S, H2 t! a! a2 B
Chapter 4: Working with High-Capacity Vectors
% `# J% X! u+ N% V4 S3 t8 g# e2 kChapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose# M; w6 D( D2 W1 d
Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA$ [3 |8 ^, a7 l7 k. |, p h
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells0 H) g: S2 L0 P7 }2 k
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
; M4 i0 H; O: L1 F. d8 V0 pChapter 9: Preparation of Radiolabeled DNA and RNA Probes) p" O H' [2 n7 J7 f
Chapter 10: Working with Synthetic Oligonucleotide Probes. o5 a K. K: ~) s5 B- t
Chapter 11: Preparation of cDNA Libraries and Gene Identification
2 _0 B: p: o4 J2 v$ pChapter 12: DNA Sequencing3 y9 _( a2 a3 Q/ o) K* A: ^$ G0 h# }
Chapter 13: Mutagenesis
$ Q) ^( D: K* n% N! B" _Chapter 14: Screening Expression Libraries$ ~2 ~9 V0 P; {8 ]0 i- V- F
Chapter 15: Expression of Cloned Genes in Escherichia coli
) Z$ [; j) T5 QChapter 16: Introducing Cloned Genes into Cultured Mammalian Cells X( T; b2 c' c7 I
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells9 q+ _& P; Y0 y/ `3 G
Chapter 18: Protein Interaction Technologies# e! m1 s% A$ O, {, x. \
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