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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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& X7 T1 }6 ]# I7 lTable of Contents
4 [3 G# E& @: P: w+ KChapter 1: Plasmids and Their Usefulness in Molecular Cloning; p5 N8 v, v# P8 @# `2 B
Chapter 2: Bacteriophage and Its Vectors5 D8 u4 k+ }9 Z4 r* c- v
Chapter 3: Working with Bacteriophage M13 Vectors
* v2 f; g T2 E N OChapter 4: Working with High-Capacity Vectors9 k4 k' Y" O- E" _, g/ K3 l$ w
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
) b+ W' A, ~3 UChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA/ e. m% Y! ]0 I
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells+ L4 d% r) p+ @. i) S* _/ [
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
3 v1 L0 [. N i1 O+ ZChapter 9: Preparation of Radiolabeled DNA and RNA Probes( K' i/ l& A8 Z
Chapter 10: Working with Synthetic Oligonucleotide Probes! s# n2 E9 _" y! z0 x) f
Chapter 11: Preparation of cDNA Libraries and Gene Identification
2 L* D. q& C$ m) mChapter 12: DNA Sequencing
+ [* _/ I. r, S% r& _3 b2 z" GChapter 13: Mutagenesis" e1 _) O0 H, s Z
Chapter 14: Screening Expression Libraries
- {+ F0 P: h. \* x- IChapter 15: Expression of Cloned Genes in Escherichia coli
! }' L. ~3 h) c1 f1 `Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells# h6 Q, P: m7 j: t9 {- a6 s
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells9 W# u) r: w1 ^. z: H
Chapter 18: Protein Interaction Technologies
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