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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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Table of Contents
2 B* S( G) B2 U. UChapter 1: Plasmids and Their Usefulness in Molecular Cloning
6 w5 E& a5 J$ I7 ?/ F( Q9 L5 UChapter 2: Bacteriophage and Its Vectors
0 f6 S) I) m1 X! r7 S( X9 zChapter 3: Working with Bacteriophage M13 Vectors! U2 Q% k( q ?6 l
Chapter 4: Working with High-Capacity Vectors
# C0 v- y2 |5 n1 I0 ]4 f# X) `Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
6 N1 \* X! N9 m0 H. t: R# PChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
$ x) ]% H( S% UChapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells7 p4 k6 g. D8 q1 K. m2 N( C
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction. h3 a7 y9 ?; ^! x7 W
Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
2 r6 S3 Y5 c/ o, v6 h. J; f# g) n/ v5 UChapter 10: Working with Synthetic Oligonucleotide Probes
" o6 e, C: j" l# P8 J# ^Chapter 11: Preparation of cDNA Libraries and Gene Identification
$ l: j3 n( J+ a) vChapter 12: DNA Sequencing
0 j. a1 D( u& a6 _& kChapter 13: Mutagenesis
0 a) \2 T! \# GChapter 14: Screening Expression Libraries
( U9 x: O/ u# a" K/ i2 EChapter 15: Expression of Cloned Genes in Escherichia coli9 ]3 G. j" u& S: o4 ^
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
4 a0 {% a! {9 X9 xChapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
: g' Y: z, U5 F: k! sChapter 18: Protein Interaction Technologies L7 E; s. N. `9 \0 b ? w$ e
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