请教一下，大家将肿瘤干细胞分选出来后怎样抑制其分化？

暮色英雄

请教一下，大家将肿瘤干细胞分选出来后怎样抑制其分化？

daviddow

放在冰上

ly200101042

我们这分选出来以后放在特定的培养基里# T, ]5 t) {5 E: d5 d+ u
呵呵 个人觉得采用养胚胎干细胞的KSR养肿瘤干细胞效果不错啊

leisong12

What is KSR, would you explain it in detail.

conanthird

leisong12 发表于 2011-5-17 22:37 % v# l* q1 @2 l" j3 `
What is KSR, would you explain it in detail.

! o8 N' w  u5 F! n: o9 _KnockOut Serum Replacement is used to support the growth of undifferentiated embryonic stem (ES) and human induced pluripotent stem (iPSC) cells in culture.
( O1 ^: w+ ?- d& m9 m. U( r1 COne of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to use. In addition, each lot of FBS is different from the last one, or the next one, so screening is a continuous task.
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% s6 j0 [6 B9 G2 z# D' K& G: A# GSince FBS screening is laborious and costly, once a suitable lot has been identified, a sufficient quantity is purchased and stored to meet laboratory needs. This ties up both laboratory funds and storage space. Additionally, there are issues surrounding hemoglobin and endotoxin levels in FBS. All of the drawbacks of serum supplemented ES cell culture, including heat-inactivation of FBS, can be alleviated with the use of KnockOut™ SR, Serum Replacement for Embryonic Stem Cells and Human Induced Pluripotent Stem Cells. / G. a% y6 ^# V. I
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture.
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0 q  Y, K' a5 x7 @$ yhttp://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Stem-Cell-Research/Embryonic-Stem-Cells/ESC-Stem-Cell-Culture-Media-Reagents/knockout-serum-replacement.html
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conanthird

leisong12 发表于 2011-5-17 22:37   S5 M" F1 \0 f5 D
What is KSR, would you explain it in detail.

  J$ d0 C/ ~* K8 W* S7 t4 K" N
KnockOut Serum Replacement is used to support the growth of undifferentiated embryonic stem (ES) and human induced pluripotent stem (iPSC) cells in culture.
( J4 [7 B& t0 j' M+ ?) QOne of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to use. In addition, each lot of FBS is different from the last one, or the next one, so screening is a continuous task. & C0 Y# X) c! Z4 I

* R% }+ t! x3 @1 `9 ^& F# M+ _- VSince FBS screening is laborious and costly, once a suitable lot has been identified, a sufficient quantity is purchased and stored to meet laboratory needs. This ties up both laboratory funds and storage space. Additionally, there are issues surrounding hemoglobin and endotoxin levels in FBS. All of the drawbacks of serum supplemented ES cell culture, including heat-inactivation of FBS, can be alleviated with the use of KnockOut™ SR, Serum Replacement for Embryonic Stem Cells and Human Induced Pluripotent Stem Cells. 1 }+ ^. m9 i) Y* _
& J5 P9 C8 i1 ?: f3 w+ p& U
KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture.. k9 P% {/ r+ S- }6 h  K
6 ?' R6 x9 C3 Y( A% k
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Stem-Cell-Research/Embryonic-Stem-Cells/ESC-Stem-Cell-Culture-Media-Reagents/knockout-serum-replacement.html
" N3 r% X5 v4 {$ [5 {& k$ }, D+ }4 }3 ]% ~8 Y

暮色英雄

conanthird 发表于 2011-5-18 09:25   i% N0 R! q/ U' `( p7 S/ j
KnockOut Serum Replacement is used to support the growth of undifferentiated embryonic stem (ES) a ...

* h/ ~6 i, @7 }6 f6 VOK,谢谢你了，我去看看这种培养基成分

jamquit

我用的无血清还是分化了 选的时候细胞放了很久而且不是冰上 选出的细胞数量少 分析了一下可能有好几个原因 下次分选要注意了

wxiouno4

据我所知,ksr好像不能看到成分

cyumin123

求楼主分选过程，帮忙指导一下！