ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

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. X) S& p/ @! Q7 \Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
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$ v% n' H4 s% I# G3 \* X/ c& oIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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0 [7 @- a2 I/ m$ d2 G- C( aChapters         Title
. Y9 J7 D1 ~3 U/ O1         Introduction* S( q! K. l  a/ B* t% q3 m! L5 j
2         Design and Equipment for the Cell Culture Laboratory
2 C; z2 |3 E( h3 J3 [3         Safety Aspects of Cell Culture* K- k* U: [9 t
4         Sourcing of Cell Lines0 f0 r  O4 M* ^
5         Cell Types & Culture Characteristics4 W% F% i" b1 o: v
6         The Cell Environment (including types of culture medium)
0 ?" K1 K" Q0 b, @. _+ M7         Cryopreservation and Storage of Cells
+ F- ?; P$ p/ z3 l: ?8         Good Cell Banking Practices
; b! L& {% Y& l8 o/ a9         Quality Control Considerations in Cell Culture
$ b; A& C1 M# s1 a9 Y10         Authentication of Cell Lines3 M2 R5 L4 T; x9 x0 R$ C. x
11         Alternative Culture Systems
) d8 n2 ^( A. A9 ?1 j12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture
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Protocols         Title
! m) D/ D% U3 G; g/ C6 r) R1         Aseptic Technique and Good Cell Culture Practice4 R1 z9 M. b: D, X% k. z, g
2         Resuscitation of Frozen Cell Lines$ l! V; K  T. `- c
3         Subculture of Adherent Cell Lines0 L3 j. z( v6 }2 x2 M
4         Subculture of Semi-Adherent Cell Lines. \4 B  B4 }6 W$ `- N8 {6 z
5         Subculture of Suspension Cell Lines( D4 \' A1 ~1 F- f- I, k/ {
6         Cell Quantification
5 A' `2 D3 U. f6 I) d4 L7         Cryopreservation of Cell Lines9 y" l4 g* i- Z- a- _. o$ M
8         Testing for Bacteria and Fungi in Cell Cultures
8 O# k* l4 R+ {9         Testing for Mycoplasma by Culture Isolation
( i3 M' ^- e& C5 |% F; Z% n10         Testing for Mycoplasma by Indirect DNA Stain
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' z6 r8 t8 [0 t0 bGlossary of Terms
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tjbone

学习谢谢了！！！！！

langziforever

非常感谢！

hormone008

good
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cc1986

学习学习

henryjia

GOOD

hany2007

谢谢分享

aminhair

看看

aminhair

晕，sigma的

bllx58

太好了，感谢楼主