ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition0 E# X: J, l3 G0 C) h7 J9 j
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In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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Chapters         Title/ T8 E& }. E: Q# z9 x& Q
1         Introduction
5 L: G- g' a5 N5 h2         Design and Equipment for the Cell Culture Laboratory4 c, z  n, e' p* S  L1 ~8 R
3         Safety Aspects of Cell Culture
+ q8 z' f8 m- G" D4         Sourcing of Cell Lines. H7 n* s9 [- o9 i' O- B# r2 J# `
5         Cell Types & Culture Characteristics% u+ }# f  ~, V2 a
6         The Cell Environment (including types of culture medium)
9 B# S4 J0 L" `7 R/ [7         Cryopreservation and Storage of Cells3 T% e6 N. L4 i- k$ o$ @( I3 F2 s0 o
8         Good Cell Banking Practices( ]4 ?5 V; k$ n
9         Quality Control Considerations in Cell Culture
. b, g! o. R1 L: s# a5 k  `  k10         Authentication of Cell Lines# G9 u# w& Y$ |# l, f
11         Alternative Culture Systems" @" x# ?( T% \: \& h
12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture+ J3 T0 W: Z; j1 \. {: n3 @, L

4 c2 L4 o) c% Y0 G0 b) ?Protocols         Title3 T; {) b2 B: ]2 M" `9 a# _
1         Aseptic Technique and Good Cell Culture Practice
# z, D+ o) d2 O! j. p  l! r8 n  E2         Resuscitation of Frozen Cell Lines
& C! n5 h. e( r* @( N% ^3 Z3         Subculture of Adherent Cell Lines
4 U5 m) v! C, u  ?4         Subculture of Semi-Adherent Cell Lines
2 k8 q6 C) [2 S8 a) q" X  A2 v5         Subculture of Suspension Cell Lines( U* Q. `! b8 Y1 p8 p* D/ W! A
6         Cell Quantification
8 k: T: b: @6 l* ~- x+ I! Q7         Cryopreservation of Cell Lines
- h& m9 A: B: [2 h& Q5 C8         Testing for Bacteria and Fungi in Cell Cultures
+ ~5 n$ C' c( y; w4 n& s) s, j3 h9         Testing for Mycoplasma by Culture Isolation* c- v5 i! @" K* A& @! H
10         Testing for Mycoplasma by Indirect DNA Stain
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Glossary of Terms5 r- P5 p2 p  b3 }0 _
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tjbone

学习谢谢了！！！！！

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学习学习

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晕，sigma的

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太好了，感谢楼主