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求助翻译 [复制链接]

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楼主
发表于 2011-9-22 20:02 |只看该作者 |倒序浏览 |打印
Our electron microscopy showed that although the mouse oocyte  ?) P* C1 v3 R2 s" ?- [# N) q
had a wide perivitelline space and few short cell junctions on the
' f4 H& k! ^4 O2 `4 tegg surface, the rabbit oocyte showed no perivitelline space but numerous# y6 Z' u  y, K  [& e; I
long gap junctions that projected deeply into the egg cortex.
0 a- K0 A: ]* ]As a matter of our experience, it was much more difficult and took8 U8 C& F# Z+ v$ v' y
much longer time pipetting to remove cumulus cells from rabbit than' L( e  U  g9 O) f7 V5 [/ ?: G- H8 j
from mouse oocytes. Shear stress generated with use of a rotating* T! K, k! I% i1 V; [
wall vessel or by pipetting induced stress-activated protein kinase/- ], f# L% E% D! F* A
jun kinase phosphorylation that caused apoptosis in mouse embryos: E# F; F5 a1 b# ^; c
(41, 42). It is therefore deduced that because the deep-set junctional
" o0 d/ {, ^; ecomplexes firmly anchored the oocyte and corona cells, although the7 G# C- e/ r$ T: U/ T
removal of corona cells damaged plasma membrane of rabbit oocytes,; r- A$ S* o! F' @& @6 V
leading to their degeneration or lysis, the long-time intensive: V" c" z% i: [* r
pipetting during cumulus denudation induced oocyte apoptosis and1 [  |0 C  F) k. j$ r
inability to mature in vitro.

. Y: {- E9 I) Z2 v8 ^; C+ j$ ]: C请问黄色字幕这两句怎么翻译呢?谢谢; G6 ]( K2 E3 [9 j3 i; ^# x

1 g% j! g( |3 D1 _1 s) H" D

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发表于 2011-9-22 20:28 |只看该作者
空心壁容器旋转时或移液管吹打时产生的剪应力诱导胁迫激活的蛋白激酶/jun激酶磷酸化,从而导致小鼠胚胎凋亡。因此,研究人员推测这些根深蒂固的功能性复合物牢固锚定在卵母细胞和冠状细胞上,尽管移除冠状细胞将破坏兔卵母细胞的细胞膜,导致卵细胞退化或裂解,但是若是在脱去卵母细胞外的卵丘细胞过程中长时间用移液管强力吹打,会诱导卵母细胞凋亡,并让它们在体外丧失成熟的能力。
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发表于 2011-9-23 09:37 |只看该作者
回复 naturalkillerce 的帖子4 T+ h! p2 n- {
% H7 E& v; |" r/ s0 u  J
多谢,版主
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