
- 积分
- 164
- 威望
- 164
- 包包
- 1030
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Our electron microscopy showed that although the mouse oocyte ?) P* C1 v3 R2 s" ?- [# N) q
had a wide perivitelline space and few short cell junctions on the
' f4 H& k! ^4 O2 `4 tegg surface, the rabbit oocyte showed no perivitelline space but numerous# y6 Z' u y, K [& e; I
long gap junctions that projected deeply into the egg cortex.
0 a- K0 A: ]* ]As a matter of our experience, it was much more difficult and took8 U8 C& F# Z+ v$ v' y
much longer time pipetting to remove cumulus cells from rabbit than' L( e U g9 O) f7 V5 [/ ?: G- H8 j
from mouse oocytes. Shear stress generated with use of a rotating* T! K, k! I% i1 V; [
wall vessel or by pipetting induced stress-activated protein kinase/- ], f# L% E% D! F* A
jun kinase phosphorylation that caused apoptosis in mouse embryos: E# F; F5 a1 b# ^; c
(41, 42). It is therefore deduced that because the deep-set junctional
" o0 d/ {, ^; ecomplexes firmly anchored the oocyte and corona cells, although the7 G# C- e/ r$ T: U/ T
removal of corona cells damaged plasma membrane of rabbit oocytes,; r- A$ S* o! F' @& @6 V
leading to their degeneration or lysis, the long-time intensive: V" c" z% i: [* r
pipetting during cumulus denudation induced oocyte apoptosis and1 [ |0 C F) k. j$ r
inability to mature in vitro.
. Y: {- E9 I) Z2 v8 ^; C+ j$ ]: C请问黄色字幕这两句怎么翻译呢?谢谢; G6 ]( K2 E3 [9 j3 i; ^# x
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