求助 谁有SRY基因组序列

yangpan521

  最近想做小鼠的雌雄鉴定 ，但没有SRY基因组序列 ，有时新手怕引物设计不好，所以求助谁有相关的的文献请赐教

goodboy

小鼠的雌雄鉴定？直接观察不就可以了吗？为什么要做SRY基因鉴定呢？不是很麻烦吗

yangpan521

有用的  回头告诉你

279042256

回复 yangpan521 的帖子* S) w& [& y1 F$ m+ D! P6 w7 `
) Y; u0 y3 Q9 ~; s+ T' n
Mus musculus sex determining region of Chr Y (Sry), mRNA
# U) }" p/ T$ k# ]. y: ~7 w' tORIGIN      2 s" |6 [* |5 {# C3 C
        1 atggagggcc atgtcaagcg ccccatgaat gcatttatgg tgtggtcccg tggtgagagg( w; M2 S5 z# M" J  D
       61 cacaagttgg cccagcagaa tcccagcatg caaaatacag agatcagcaa gcagctggga
" y# k  C( b! T5 s7 W2 E- ~      121 tgcaggtgga aaagccttac agaagccgaa aaaaggccct ttttccagga ggcacagaga3 r) Y2 X! J9 W( C! P0 ]; D0 r* t8 b
      181 ttgaagatcc tacacagaga gaaataccca aactataaat atcagcctca tcggagggct$ z$ P( A3 r, |1 R( c& @; B
      241 aaagtgtcac agaggagtgg cattttacag cctgcagttg cctcaacaaa actgtacaac2 m& T& F  k  R: S/ ^1 |
      301 cttctgcagt gggacaggaa cccacatgcc atcacataca ggcaagactg gagtagagct( c1 B' p% P: P
      361 gcacacctgt actccaaaaa ccagcaaagc ttttattggc agcctgttga tatccccact
4 G* L1 L4 E: \* y: r      421 gggcacctgc agcagcagca gcagcagcag cagcagcagc agttccataa ccaccaccag0 T) u% x, ~/ |0 L/ l/ ~7 q8 z
      481 cagcaacagc agttctatga ccaccaccag cagcagcagc agcagcagca gcagcagcag
6 M: [2 B' m$ c5 p" }) E: D5 G      541 cagttccatg accaccacca gcagaagcag cagtttcatg accaccacca gcagcaacag
: e. N- l5 j' l6 h; ^  A      601 cagttccatg accaccacca ccaccaccag gagcagcagt tccatgacca ccaccagcag  z5 i9 H& I( G/ F. ?$ }3 s2 w7 d
      661 caacagcagt tccatgacca ccagcagcag cagcagcagc agcagcagca gcagttccat
4 S/ g  m: _! ~' Y      721 gaccaccacc agcagaagca gcagttccat gaccaccacc accaccaaca gcagcagcag3 r; h* z" B3 Y5 D" A; c3 M
      781 ttccatgacc accagcagca gcagcagcag ttccatgacc accagcagca gcagcatcag
' e3 b2 D6 O, o& f. n( h      841 ttccatgacc acccccagca gaagcagcag ttccatgacc acccccagca gcaacagcag# ?  c" C5 m+ K! G" T
      901 ttccatgacc accaccacca gcagcagcag aagcagcagt tccatgacca ccaccagcag* r( i' C% I+ C1 H$ e
      961 aagcagcagt tccatgacca ccaccagcag aagcagcagt tccatgacca ccaccagcag
$ z5 i! W  U7 g  {     1021 caacagcagt tccatgacca ccaccagcag cagcagcagc agcagcagca gcagcagcag
4 x' F6 A; b& J$ z4 V. `     1081 cagttccacg accagcagct tacctactta ctaacagctg acatcactgg tgagcataca, n. a- G+ Y. ~. p% S$ r
     1141 ccataccagg agcacctcag cacagccctg tggttggcag tctcatga
+ Y1 d) N: v9 W1 {8 E//

279042256

回复 yangpan521 的帖子6 N1 q& q; G8 t3 t( e: ~; p
9 P3 a0 }0 }" q! a" R) |7 a/ s
sex-determining region Y protein [Mus musculus]
1 U3 W& A: N: M8 n/ EORIGIN      
6 M* t8 U$ k/ @6 g2 T0 @7 S5 O        1 meghvkrpmn afmvwsrger hklaqqnpsm qnteiskqlg crwkslteae krpffqeaqr
0 |. X. `) x7 x* b  }       61 lkilhrekyp nykyqphrra kvsqrsgilq pavastklyn llqwdrnpha ityrqdwsra& D4 [$ i5 l. Z4 W* R, ?# t9 D
      121 ahlysknqqs fywqpvdipt ghlqqqqqqq qqqqfhnhhq qqqqfydhhq qqqqqqqqqq
) X% s( F- d5 }! b* G0 p      181 qfhdhhqqkq qfhdhhqqqq qfhdhhhhhq eqqfhdhhqq qqqfhdhqqq qqqqqqqqfh0 P) p5 H- k" e
      241 dhhqqkqqfh dhhhhqqqqq fhdhqqqqqq fhdhqqqqhq fhdhpqqkqq fhdhpqqqqq3 g: B& I* N5 r) d
      301 fhdhhhqqqq kqqfhdhhqq kqqfhdhhqq kqqfhdhhqq qqqfhdhhqq qqqqqqqqqq
9 R4 D% U+ L1 v      361 qfhdqqltyl ltaditgeht pyqehlstal wlavs
# l* ], N2 E! t; N: H8 G; `8 ]

279042256

回复 yangpan521 的帖子
5 ^3 B, @2 G' A  t4 e
. E% g1 O; Y5 O3 L0 I* K( }0 r! \7 n
Mus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J1 `+ C3 t1 o% k9 z  Z6 b

" F" x% u, U0 B; D% D4 s" O& O  DNCBI Reference Sequence: NC_000087.6& m4 b6 ?3 O1 f* p$ W% c* z
4 i  a3 H1 P7 c% P/ `& x$ r
GenBankGraphics
. L$ f& X& f% _: Q! \- [% k! J: O9 u4 `* W* i" |) N
! z4 ^' h4 P" s8 a/ @, ]
; c( T+ o, s" k; j% A( k

4 c% i( G* @$ S8 J. n>gi|149361526:c1919568-1918381 Mus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J% H2 Z# |$ w* r
ATGGAGGGCCATGTCAAGCGCCCCATGAATGCATTTATGGTGTGGTCCCGTGGTGAGAGGCACAAGTTGG
2 H- _# E: g) \, {CCCAGCAGAATCCCAGCATGCAAAATACAGAGATCAGCAAGCAGCTGGGATGCAGGTGGAAAAGCCTTAC* ]. I( p9 P2 ^: X4 Y7 z8 ]( D
AGAAGCCGAAAAAAGGCCCTTTTTCCAGGAGGCACAGAGATTGAAGATCCTACACAGAGAGAAATACCCA
( N) R5 p/ Y% i1 L) H/ y% d% p6 O+ ZAACTATAAATATCAGCCTCATCGGAGGGCTAAAGTGTCACAGAGGAGTGGCATTTTACAGCCTGCAGTTG; w% G9 I4 C; t% k
CCTCAACAAAACTGTACAACCTTCTGCAGTGGGACAGGAACCCACATGCCATCACATACAGGCAAGACTG
- K- k& s& o; s& {: ^% m% h9 ^: AGAGTAGAGCTGCACACCTGTACTCCAAAAACCAGCAAAGCTTTTATTGGCAGCCTGTTGATATCCCCACT! D  M, m! e6 [- W& B
GGGCACCTGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATAACCACCACCAGCAGCAACAGC
# u8 s! o& @. e9 J1 F7 SAGTTCTATGACCACCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATGACCACCACCA9 j5 m7 l* R, A$ O8 f4 z* r4 U
GCAGAAGCAGCAGTTTCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCACCACCACCAG
( D* l0 @6 {) v& vGAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGC
; z- n* R& U" j8 BAGCAGCAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCACCACCACCACCAACA
' E1 B( n$ [  e& E* d  e  ^' p% EGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCATCAG& G; `+ k/ U3 ~# Q+ h; }7 v7 V; [
TTCCATGACCACCCCCAGCAGAAGCAGCAGTTCCATGACCACCCCCAGCAGCAACAGCAGTTCCATGACC% c) b1 y; r6 @0 z; }! Y$ P% [% f2 g
ACCACCACCAGCAGCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCA2 k7 n) g0 h# X4 `& s
CCACCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCAGCAG
. M. O" V6 ?/ h4 ~0 T. cCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCACGACCAGCAGCTTACCTACTTACTAACAGCTG- C3 ^, c4 B8 O
ACATCACTGGTGAGCATACACCATACCAGGAGCACCTCAGCACAGCCCTGTGGTTGGCAGTCTCATGA
1 i. U  ^- t5 h( l3 p' K' C; R- e  [7 o. W# s9 r7 k" |

279042256

小鼠性别鉴定技术很成熟，找点Y特异基因设计个引物跑个PCR就行，很简单，但是要注意防止假阳性和假阴性，两个对照一定要做好

yangpan521

谢谢各位

yangpan521

回复 279042256 的帖子
* ?) P: V$ z) _* G) S; K( \
' h+ W. _, t4 a2 |( ?1 `1 n4 J我是要鉴定囊胚 ，请问如果囊胚数量少到1,2个时还能鉴定吗？

279042256

是1-2个囊胚还是1-2个细胞啊，小鼠上没做过，牛上可以，切割下几个细胞就可以做鉴定的