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发表于 2011-11-17 11:36 |只看该作者 |正序浏览 |打印

本帖最后由细胞海洋于 2011-11-17 23:41 编辑
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PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press# z5 \0 k9 d/ g/ ]* L% _; F3 a
, Q6 p. ?: B5 N* n' {  u
主要内容如下：

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1, G7 F! k! v/ J1 X3 G+ M! N
Christina Philippeos, Robin D. Hughes, Anil Dhawan, 4 s( U7 A7 g2 @
and Ragai R. Mitry
  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate 5 F+ E) ]& _' c! l( q- N  r+ _
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
  3  Isolation, Cultivation, and Application of Human Alveolar
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, - A9 Y) c: G' z' b( T6 W
Hanno Huwer, and Claus-Michael Lehr
  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43. j. J/ ]- S# w$ [" Y
Peyman Björklund and Per Hellman, T; V9 M, p0 z9 S6 C
  5  Functional Analysis of Human Islets of Langerhans Maintained , v1 r" p( t9 d) n
in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55
Shanta J. Persaud, Bo Liu, and Peter M. Jones 1 X" S+ _2 b/ u; A+ F% t
  6  Conversion of Non-endocrine Human Pancreatic Cells , J& C6 t* J& b: r% s% N
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73# N2 F% M0 E) Z. s# Y
Min Zhao and Guo Cai Huang% `7 a% c2 w3 a' A+ D& Q
  7  Evaluation of Cytochrome P450 Activities in Human
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87
María José Gómez-Lechón, Agustín Lahoz, José V. Castell, . O4 n7 `; c2 U
and María Teresa Donato
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, 0 Q, b4 ~6 n/ c/ l( {1 A( {
Peter Büchler, Michael Schoenberg, Jörg Kleeff,
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy,
Jan Hengstler, and Andreas K. Nussler 7 f* \% t) r" ^! c5 m. d. c
  9  Preclinical Testing of Virotherapeutics for Primary and Secondary
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121
Martina Zimmermann, Timo Weiland, Michael Bitzer, 1 g; N  p& f6 E! x& y" a3 y/ @5 s
and Ulrich M. Lauer
10  Isolation and Functional Studies of Human Fetal Gastric % s* |" b& F* n. Q; @0 ~' M
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137; ]3 C" i1 @) S% ~5 t+ g3 ]- K
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard2 R* {! N7 c' F7 R
11  Isolation, Characterization, and Culture of Normal Human 9 L+ K7 F1 S; A- n  J
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1576 y+ v9 U( m! p
Jean-François Beaulieu and Daniel Ménard, S& V1 C8 }2 D
x Contents1 p* ~, ^8 y* i& w3 @# A
12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells 6 n, r9 D! O" t3 v
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175, I- `% m; @- Y$ N
Claire C. Sharpe and Mark E.C. Dockrell
13  Glomerular Epithelial and Mesangial Cell Culture # K: _! r/ t6 T9 h
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187# }8 f" M4 Q& D+ G# ^. Y
Heather M. Wilson and Keith N. Stewart% K4 p  y! b0 l2 C, P& W
14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203( e4 C9 e1 l" l
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
15  Primary Culture of Human Adipocyte Precursor Cells:
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2159 W& V4 i3 Q8 N) h0 }6 Z
Thomas Skurk and Hans Hauner
16  Primary Culture of Ovarian Cells for Research on Cell Interactions 1 h/ |4 V( @6 D0 c/ M
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227) q% q) T9 z9 z! u  R
Jerzy F. Galas) d$ O* I& e& R7 L* b# W" Y. G
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251* @, Z+ h4 H" d  p# h1 D/ L# `% c
Diane Proudfoot and Catherine Shanahan
18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
Richard C.M. Siow
19  Human Peripheral Blood Mononuclear Cell Culture for Flow
Cytometric Analysis of Phosphorylated Mitogen-Activated
Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, 1 F) [3 E( w& a5 E" {
and Dimitrios P. Bogdanos7 e8 B% v8 a' ]6 ^/ V- c
20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
Haibin Su, Maria Serena Longhi, Pengyun Wang,
Diego Vergani, and Yun Ma
21  Human Chondrocyte Cultures as Models of Cartilage-Specific " m! d" F; _* [$ r! l( F; c" v- u
Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301: c# ?. e' U- j
Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, / b- u  z( N+ I5 n( Y
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring, i- K& Y9 O3 x9 w6 V  X
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337# ^) r  g1 e! A7 _) U) G9 C* @
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, ! d' l' z* l9 ]) G/ o) \# R
and James A. Gallagher
23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
Ankita Agrawal, James A. Gallagher, and Alison Gartland
24  Effects of Temperature Generated from the Holmium: YAG Laser
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, 4 ~* ^: ?" T3 c8 X8 Y/ G2 K$ S
Ian D. McCarthy, and Al-Shymaa M. Hafez
25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  3859 h* x: k' K+ q& w0 e- a
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia' p% z/ J7 q4 N6 L. c9 |% A! S
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393
Robert Thomas and Elizabeth Ratcliffe/ Q$ c3 F9 _/ ~( P& _  y  u* s
27  Culturing and Differentiating Human Mesenchymal Stem Cells
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  4077 m  {4 J* \( R# B' E" s0 _
William R. Otto and Catherine E. Sarraf
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  427  u- k( l. J2 _. ^9 j2 c! Q
, R, `" K* N* R. ^6 X
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