PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press
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8 i6 x. R. b% u0 r8 o主要内容如下：$ g2 ^+ C1 \1 |
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Contents% T8 f! m0 W' P2 _9 H
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii6 D; f) B% O6 l# d
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi& a$ v( k/ Q# E2 O1 V1 C5 b% {- \
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1$ r) L$ h4 l; Z0 M
Christina Philippeos, Robin D. Hughes, Anil Dhawan, , V( e7 B# f# K
and Ragai R. Mitry
* F7 I9 A! I: c2 p" ]9 [  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate . ]# d; n7 }; Z5 B1 Y
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15" L" X! P  n1 D4 E
Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
9 c+ y7 F: E" z  3  Isolation, Cultivation, and Application of Human Alveolar 3 ?! D5 q8 l& Y  S) c
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
) ^4 K! r. C; K3 J+ ^Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, 2 g+ z4 S9 V& f% s; X
Hanno Huwer, and Claus-Michael Lehr8 @4 ^1 J! M# u- l8 L$ p
  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43
. `, t* I& w, nPeyman Björklund and Per Hellman4 p# x; \7 |. g: l+ O
  5  Functional Analysis of Human Islets of Langerhans Maintained & W2 R5 G  ]& x# i+ _! S8 v3 L
in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55+ C. z: y8 c( E; j& ?
Shanta J. Persaud, Bo Liu, and Peter M. Jones - S' p! k/ E% {7 v; T
  6  Conversion of Non-endocrine Human Pancreatic Cells
. T& Z2 r% _. q) T' v. p  |- `to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73
+ F" B0 \  S7 V6 k0 _9 VMin Zhao and Guo Cai Huang
+ [8 |, P) _/ B; z  7  Evaluation of Cytochrome P450 Activities in Human ! _- {0 [* e) A) c, q" v0 a
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87, U5 o  m+ g& _: X. B9 K8 K- K+ D
María José Gómez-Lechón, Agustín Lahoz, José V. Castell,
9 H2 u! y6 |$ K$ j  A) b8 g7 R- vand María Teresa Donato5 \" h. {+ T- M+ U; F& v9 A' v
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99; K; h6 X6 S$ G, ~9 w5 D0 f6 t! w+ c. A
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka,
) \( h, J* _- ?Peter Büchler, Michael Schoenberg, Jörg Kleeff, 7 V) O  W7 t6 W8 m$ K
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, 4 u, H  o! l* r- u7 O* M* A
Jan Hengstler, and Andreas K. Nussler
% w/ K% `% J# o6 @" f" U  9  Preclinical Testing of Virotherapeutics for Primary and Secondary & o; k  `9 u; F+ [! n
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121: C& ~& n% w. e, H3 R, M; a+ x
Martina Zimmermann, Timo Weiland, Michael Bitzer, 3 l1 t5 }+ B4 f8 s& v0 b
and Ulrich M. Lauer
2 j4 C" u; m7 n10  Isolation and Functional Studies of Human Fetal Gastric 1 W- B9 y$ X8 @" z/ L+ m% I
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
: m  W* t' @" C' Y# E1 f# nPierre Chailler, Jean-François Beaulieu, and Daniel Ménard
7 L4 t, o. q3 J& X" X: q11  Isolation, Characterization, and Culture of Normal Human / J: j! b2 {: D( s& I1 L) a
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
: u( C; b# [, l* \5 Z5 [; _Jean-François Beaulieu and Daniel Ménard
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12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells
1 r" I! v7 u. k* Tand Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175
1 [: t: o2 s$ Y+ C0 Y, B9 S, vClaire C. Sharpe and Mark E.C. Dockrell
3 r8 |3 K% _. d5 u13  Glomerular Epithelial and Mesangial Cell Culture
1 p0 h0 r) K* N# |& u# Land Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
- M/ h' X! ~1 AHeather M. Wilson and Keith N. Stewart
/ m4 |9 r- {( n6 ^* V$ u9 x14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203
3 g) p  R( q3 L6 q# H, Q1 k! VKirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
/ I3 D8 p4 ^! C+ c2 H" ~' i( ?3 @15  Primary Culture of Human Adipocyte Precursor Cells: 6 A+ s0 \0 Y& n* O
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215( Y% ^) o, l+ e. P) Z4 q
Thomas Skurk and Hans Hauner
6 n( k4 ^% z. v: ?: ]* ^' h16  Primary Culture of Ovarian Cells for Research on Cell Interactions
3 w+ K  O5 I: t3 U; [2 Ein the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2278 H! x) K& P) d' D: C2 Z3 R
Jerzy F. Galas/ K& I( V* Q2 b2 i
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
. X% J( p2 d2 i/ |' `- aDiane Proudfoot and Catherine Shanahan
* \9 v" |5 |& I4 \/ z+ V" F18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
( E7 T, K" Q. B* r8 H7 |Richard C.M. Siow0 O. Z- b# r7 Z5 L* j' }6 T# ^
19  Human Peripheral Blood Mononuclear Cell Culture for Flow
6 V# H7 d: O- F! U( r6 d! DCytometric Analysis of Phosphorylated Mitogen-Activated
4 k* h9 w  A# l$ s0 CProtein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275; n9 X4 w  E: t2 Z" `) j6 a
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, ( g4 U; ]! ~( A0 T
and Dimitrios P. Bogdanos
4 e/ H* k$ S; U+ r% z20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
! p- ]6 d% L5 hHaibin Su, Maria Serena Longhi, Pengyun Wang, ) n8 v9 m+ {: v5 o8 U5 Y# l
Diego Vergani, and Yun Ma6 k* p7 U6 B; \0 A/ |6 w! p( ~
21  Human Chondrocyte Cultures as Models of Cartilage-Specific
8 D0 H& b4 [  }! d) X6 L' ^Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3019 |0 K0 B" d/ ]- L
Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem,
3 I. T5 x1 E- Q  x1 LKo Hashimoto, Darren A. Plumb, and Mary B. Goldring
% @% p# Q. b: N& p" O& b4 y% R4 b22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337
# E4 o1 u; v7 I( h/ Z+ j9 iAlison Gartland, Robin M.H. Rumney, Jane P. Dillon, / {( ~' S' h5 p5 r" t; m
and James A. Gallagher
$ M$ b- m! c% E9 Q23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  3577 S6 v# ~( v; s  ~
Ankita Agrawal, James A. Gallagher, and Alison Gartland
3 E- ?& T, \, A/ o24  Effects of Temperature Generated from the Holmium: YAG Laser
" [2 ?& |" D6 u1 _on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377! H6 L% Q5 E/ m+ l# x7 n" {
Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, . o& N# o1 A$ k* |0 N3 d  ~& Y
Ian D. McCarthy, and Al-Shymaa M. Hafez $ @' l$ Y. V( i% |/ {1 y' p
25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385
9 C# m. j" t8 dAhlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia3 K* Q% Z- @! ^4 ^. V5 g
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393
8 i: ^( o5 v$ q- k  aRobert Thomas and Elizabeth Ratcliffe$ V7 I7 Y/ S9 c$ }$ ?
27  Culturing and Differentiating Human Mesenchymal Stem Cells + A! u* h4 e+ c3 @6 B7 R# ~7 j; ]8 M
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  4078 Y" J: u+ @3 T: R3 {" [, t( \* t
William R. Otto and Catherine E. Sarraf 0 R$ q2 l  P/ Q) ]8 C! ]; ~' d6 {  M) ^
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  427. n( W0 o+ U6 N7 w- Q4 b  u$ }$ j
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