PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press
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Contents
/ Z; G, `! a2 VPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
+ M$ X: [* T, f; b9 g2 o8 t1 ~% {Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi
4 z- T2 U' D# L0 q' ]  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1) U! K: h& d7 I
Christina Philippeos, Robin D. Hughes, Anil Dhawan, 2 W) r$ A' J! B6 ~
and Ragai R. Mitry: m# C6 H8 B1 U% U  n& }& T2 O
  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate
- {* A  B3 p1 D3 t' Tinto Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
: ~' M- z$ w( N: C$ |8 cLing Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
9 t. @3 B3 I: {: V  3  Isolation, Cultivation, and Application of Human Alveolar
( O6 P; t3 Q- Q! TEpithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
, m' i1 J+ B( Q6 U" T6 v2 tNicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, 1 q( P0 h  ~$ e9 I3 @, z" ]' a
Hanno Huwer, and Claus-Michael Lehr
  y: Q1 P9 }8 o3 U* U. Y  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43: ~* z3 w! u7 y- f% ^, T
Peyman Björklund and Per Hellman1 H) v  A; [: T8 |0 _0 u9 {
  5  Functional Analysis of Human Islets of Langerhans Maintained - B* ~% X6 |* N4 u% ~, C3 c
in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55; E9 T' F  X. I! z
Shanta J. Persaud, Bo Liu, and Peter M. Jones * q  y7 w+ U; D" |: H  v0 R/ y
  6  Conversion of Non-endocrine Human Pancreatic Cells . Y5 U- K2 M! \: r- G
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73  F! Y6 d. G1 v  U9 M+ I
Min Zhao and Guo Cai Huang9 a6 ~' G3 A  W/ o
  7  Evaluation of Cytochrome P450 Activities in Human
5 F6 O9 x! L2 ZHepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87
9 ~" P" N+ c8 ^% M" ^María José Gómez-Lechón, Agustín Lahoz, José V. Castell,
- ]; {. N- ~( `( {" I' ?) Jand María Teresa Donato: |  A' C2 Q5 V
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99
$ Y. t0 m; d7 W' L3 ^/ ~7 [Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka,
0 _& s% b0 K3 O5 I2 Q" pPeter Büchler, Michael Schoenberg, Jörg Kleeff,
5 B  \: c5 P3 _* i3 fWolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, + M  k' V; I) R3 k9 J. N
Jan Hengstler, and Andreas K. Nussler # N& T- V! A5 R$ H( N: Q
  9  Preclinical Testing of Virotherapeutics for Primary and Secondary - A$ R- K* q! r9 E
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1214 X, l2 c, b% d0 p7 a' d3 T* |
Martina Zimmermann, Timo Weiland, Michael Bitzer,
+ z# N1 u- d& Z! C. Xand Ulrich M. Lauer) g& t2 p6 \/ N% U/ U1 t3 c" `0 r* {
10  Isolation and Functional Studies of Human Fetal Gastric
" w; p+ o1 Q8 E1 WEpithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1374 r4 q% O' h, z
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard  K) A2 W/ k+ Q- N& n  h! A: }& h
11  Isolation, Characterization, and Culture of Normal Human   u& R% b% U3 G; N/ M
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1577 x! w4 N$ Z4 h
Jean-François Beaulieu and Daniel Ménard
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12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells ! J5 C" F' B1 m5 D  @
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175
* C9 e. O$ d+ HClaire C. Sharpe and Mark E.C. Dockrell& }4 F$ `" r: x! C  @! X) K' M
13  Glomerular Epithelial and Mesangial Cell Culture % T+ u" e& X8 H, z
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
2 c, \' L" S. ~/ mHeather M. Wilson and Keith N. Stewart4 |: W" L9 u% V9 H" {, z
14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203! F0 R* Y; r9 J! E  }% b
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried9 c- r8 Z) S2 ^
15  Primary Culture of Human Adipocyte Precursor Cells: * r6 {# i/ ^+ O8 O& M0 k
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215
, b+ j1 z% Q( e; X6 T+ xThomas Skurk and Hans Hauner
! k# [- Q0 M/ s( @: S0 ~3 Y7 i16  Primary Culture of Ovarian Cells for Research on Cell Interactions 6 B' |# r6 s1 d5 n* W
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227; W, i- e0 v4 G) b8 _
Jerzy F. Galas- }4 @/ \- q# |" x/ a
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
% o0 L; N8 D" p# ODiane Proudfoot and Catherine Shanahan
3 H0 [- F! d" h+ N2 U9 n+ d18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
: s' b5 r1 D* DRichard C.M. Siow
" `- X, r  J/ |0 q: o, Z) @8 ]( l19  Human Peripheral Blood Mononuclear Cell Culture for Flow
/ Z% i* W& w4 n, L( lCytometric Analysis of Phosphorylated Mitogen-Activated - `) v! C) y, }$ x" ]: N
Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275) w0 j3 A4 F& _3 G
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, # ?  e4 k& k  j
and Dimitrios P. Bogdanos
" H2 Q- J  u4 g" y& X: Y1 I20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
9 l8 g1 k0 F3 [7 k$ nHaibin Su, Maria Serena Longhi, Pengyun Wang,
# h+ c5 t# Z' h+ {, C; uDiego Vergani, and Yun Ma' _- B9 d, Z* m# I
21  Human Chondrocyte Cultures as Models of Cartilage-Specific . n9 c) ^3 d3 B
Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301' Y! c' v" N5 W3 F% v8 p# {+ [
Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, 7 ?6 G5 m7 @+ B' {
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring
/ F5 H* w' h! p3 @' I! j22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337" B! a; H) H8 n6 D( _: L7 t
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon,
  l# }3 x* o8 I  I! O! V& [! n) Uand James A. Gallagher
1 F: i6 ^; b! c- W  s) y8 `7 e4 f23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357, ]5 r; m6 c# M- X8 J
Ankita Agrawal, James A. Gallagher, and Alison Gartland
1 ]! q/ X- }( i) {3 H4 p24  Effects of Temperature Generated from the Holmium: YAG Laser
) p. }( a0 i6 N' S: k& H3 \on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377* l$ w& P* A! ]/ l, ?3 d
Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs,
+ L: k+ u: R: u- T& q  S' o( N9 MIan D. McCarthy, and Al-Shymaa M. Hafez
9 B2 s2 o% n6 ~: u5 ?25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385" L4 |7 a+ G1 u
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia
9 S4 b' [0 O1 D26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393  a6 G; h, y# A7 W0 q% J
Robert Thomas and Elizabeth Ratcliffe
: K* s; ]% @+ v% f0 {27  Culturing and Differentiating Human Mesenchymal Stem Cells
0 B  G5 M9 v' v3 D; l' }, E/ L) jfor Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
0 ~+ D/ b/ g% J. uWilliam R. Otto and Catherine E. Sarraf 9 p5 U# C' E& t; w6 m+ G
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  427
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