PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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/ M0 L2 ^. {6 G9 s$ c4 c METHODS   IN  MOLECULAR  BIOLOGY
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Protein Expression in Mammalian Cells' _0 G' e4 _6 `* E5 q
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Methods and Protocols# D, k* g" l, }0 D' t5 \( L
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Edited by
4 \( b8 D9 P9 dJames L. Hartley
6 p4 }, ?( P3 b2 R, ~, h+ hProtein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute, 8 S" y# E2 q9 y1 R* M3 ]! \
Frederick, MD, USA
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Springer Science+Business Media, LLC 2012
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Contents
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v
1 F7 Y9 m. z: L# _. B) f3 CContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix0 t1 a, b6 D7 d
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  18 R4 e2 \2 S, F" S8 t/ U* h5 u: f2 \+ y
James L. Hartley9 H% \. Q0 N! p* m' y4 L5 d
  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13
3 g5 J  h4 ?8 R* \8 \3 {Lucia Baldi, David L. Hacker, Carine Meerschman, 6 K/ ?* I9 [( C( o2 a* ~
and Florian M. Wurm% B) B& }; I- |  R
  3  Selection of High Expressing Mammalian Cells by Surface Display : p& t- C1 ]6 W5 A) n. O6 N
of Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27, K4 c1 \( D" `# u
Christine T. DeMaria7 `. ], ]3 p9 [, r2 i8 T" S* ?
  4  Expression of a Secreted Protein in Mammalian Cells Using & a9 d4 c# b9 g" k  x9 j4 ?
Baculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41
. n0 p2 F& S7 N* I- }Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash) L1 o  ^6 Z) j, A1 ]" E6 h: B
  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65
; N: n, v# i2 _+ \6 ?Oliver Gresch and Ludger Altrogge
7 K& j4 Q; Q2 f$ h  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  75& I! X& O9 P0 u' }
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, # a1 i' J# D" p, {% k' A
Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch$ H! A  I2 P. A% n' h1 Z
  7  Using Matrix Attachment Regions to Improve Recombinant
8 i3 i! U( [& M1 [5 Y$ b( N. w9 W( nProtein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  931 p  A9 u$ ]' F
Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, . z- ?  T; d( \3 f' t0 }, \
Pierre-Alain Girod, and Nicolas Mermod* V4 {  p; t. {0 Q& a
  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111
- N+ @* e" ]6 O0 _& GMatthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh,
) m7 C6 q$ |& O; A, eand Tina M. Sauerwald% d3 h" S7 m- Z" E1 J( b1 ?
  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene ( S1 `9 u- w" e
Expression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1256 l. E3 `, l6 k" X5 o
Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
' v: o" `, }; ~2 x* ]- H0 o10  Converting Monoclonal Antibodies into Fab Fragments for Transient
# J9 t) [- L$ T' IExpression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
0 o4 {3 u% J/ I! h% }Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, $ g8 K0 {% m2 K+ {8 N1 P
Rebecca Hamer, and Raymond J. Owens
$ E7 N: w/ T" k! c' B11  Generation of High-Expressing Cells by Methotrexate Amplification ; X" E) f# j- S) K  G0 y( r) ~( X9 }6 ^
of Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  1615 z4 w0 ~" K6 B! _) J- Q% t
Say Kong Ng
1 I/ g$ v8 u9 U12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  1739 P  z; X: L+ r7 s' Y( l3 r
Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia3 y; ^+ c! r) S7 W- o
viii Contents
: P+ Y; m7 M4 S# C* O& V9 [7 [& {13  Identification and Characterization of Protein Glycosylation Using Specific 6 d% v/ r6 ~3 `* O  |
Endo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189
- }% P- A0 V! E9 m& TPaula Magnelli, Alicia Bielik, and Ellen Guthrie
1 L; @1 R, N/ y( t4 f14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213
$ r7 y1 g7 Q6 _Renate Kunert and Karola Vorauer-Uhl2 }$ S* l9 n# B5 T2 C
15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  2272 r+ S2 Z  Q8 f7 ?+ o- r# {4 S
Takefumi Sone and Fumio Imamoto
; f( [2 G/ L: [, H( B  H) z& D16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  2517 D, F* q3 G( f) Y+ M8 v
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito7 k4 ^/ ~1 i- i$ T! K
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269
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mimimi2256

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aidiulgy9517

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Why Proteins in Mammalian Cells

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cc1986

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