PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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3 n: O! K% a. }* u3 m/ N  R3 A9 o METHODS   IN  MOLECULAR  BIOLOGY+ k9 w" q1 B( M* @

- L5 [3 T2 c: W; SProtein Expression in Mammalian Cells/ w/ P- S4 q% A/ ~/ x, Q

' U6 n) A6 m. I+ Z% ]2 }8 g5 nMethods and Protocols
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0 E, b. l" B! l. oEdited by, u7 D3 z( s# m8 @
James L. Hartley4 s& I8 j1 y6 L
Protein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute,
' R! B! f& K4 R$ }Frederick, MD, USA
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Springer Science+Business Media, LLC 2012+ `. A! H1 a+ N: i0 Y9 f

" g6 L* H0 F$ S9 h, W* lContents: ^  B9 p+ C5 U$ T
Contents! }4 B$ g5 p& @; ~" f- Z
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v, h0 D: t1 M# ^5 p$ }2 o  a
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix  f/ L3 L# j3 @) `1 Y
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
: ~# r) [: l3 h" j8 w' \' rJames L. Hartley" b* n1 P# _' L
  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13
* J' T6 s& @& X/ R8 {9 y& TLucia Baldi, David L. Hacker, Carine Meerschman,
$ e& _9 w# {7 ?# r, }and Florian M. Wurm
! s+ p6 C  b2 D1 X( @# {$ x  3  Selection of High Expressing Mammalian Cells by Surface Display
- _; M. E" |3 E; D9 tof Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27% p8 j# W: ]% H; F
Christine T. DeMaria
+ W: |  w6 F+ ?5 O  4  Expression of a Secreted Protein in Mammalian Cells Using 2 A8 r1 O4 a' b$ S, B) y4 }$ k
Baculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41# X1 z& ]! L0 i, Q1 v' A
Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash% H9 s2 N% r3 D; }' E9 V# v0 L
  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65
' B. N( |2 `" c; |Oliver Gresch and Ludger Altrogge& h  [( t- ?3 m
  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  75
0 a- a7 p$ l9 H# z8 A, PAndreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty,
# u5 ~2 f2 X0 o  Z$ k& ]- eSabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch" d1 f8 \6 E, [1 x
  7  Using Matrix Attachment Regions to Improve Recombinant & t* W# ?/ h0 u! r' B" b
Protein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  93
( O  ^$ k! A+ jNiamh Harraghy, Montserrat Buceta, Alexandre Regamey, 3 D$ K; v9 \4 p8 }( D0 W
Pierre-Alain Girod, and Nicolas Mermod* D; r. J3 s4 K: _  Q
  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111/ a# c3 v6 O7 ?5 J* ?4 P
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh,
/ i' @4 l- Z; s/ Y  R" _/ s* f: Zand Tina M. Sauerwald
. w3 f4 n9 ~2 T% A5 n- W  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene + _- h0 i& {: T1 w) M
Expression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  125
( x8 p( F# ?9 |Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
8 z6 o5 b$ @9 m8 m5 Y10  Converting Monoclonal Antibodies into Fab Fragments for Transient
; b# s# W5 j6 b8 |5 eExpression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137/ z+ y. y; n. F+ J, E
Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, ' y& [& f& N2 {$ r' R& }: |" ^
Rebecca Hamer, and Raymond J. Owens
, B  o$ m1 f% d$ [7 S; m  F4 _' g6 Q  d11  Generation of High-Expressing Cells by Methotrexate Amplification
5 H' q5 O1 {4 |$ \. h3 A$ M, Pof Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  161; u: \' W" g/ d2 k, B7 z
Say Kong Ng( l5 K  \' s: Z* S
12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  173
/ j7 f$ R$ j$ LZahra Assur, Wayne A. Hendrickson, and Filippo Mancia
6 ?/ O8 {' t; ?( U% B3 X3 |viii Contents
: e' [1 S+ U& p  D13  Identification and Characterization of Protein Glycosylation Using Specific + u! Q- n/ i  [' x" G7 {) F
Endo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189
1 u5 ~% Y1 X9 z- ?Paula Magnelli, Alicia Bielik, and Ellen Guthrie( m3 P; z7 [( a
14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213
7 I$ G9 e5 F. p7 XRenate Kunert and Karola Vorauer-Uhl
) O! c7 F% d& o15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  227
+ B% n" x1 E$ h, }. K1 o3 kTakefumi Sone and Fumio Imamoto
2 d  V) c7 w  N- V/ i" v16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  2517 ^, N! T& F. A
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito
& X5 v' c% S$ Y* ~" jIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269) O: V7 r: B. ?0 Z/ W8 @
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