PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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1 T/ g: Y4 T/ c/ k5 y) B METHODS   IN  MOLECULAR  BIOLOGY: }4 c6 V+ i" @$ i% H. `4 W
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Protein Expression in Mammalian Cells, o) J1 G  {; u# y0 Q7 E* ?/ R' ~
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Methods and Protocols
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! ^2 M& Y; z2 I) ~5 `1 }; ?Edited by
) @% E& M" R$ k$ N' r  {# b% [* WJames L. Hartley
% J! q, q: a# E2 Q8 p: ?5 P+ ~Protein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute, - j3 B- X/ @! m+ z. K
Frederick, MD, USA
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* T' J5 V9 u; q, u, X/ @Springer Science+Business Media, LLC 2012
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Contents
% S2 ~8 ^5 r5 ?4 sContents
4 n9 o; d# `2 N! n# aPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v8 b% Q. ~6 c! Y% J
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix" d0 |- j2 ?: d: v
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
; w9 \8 \, I0 c+ R3 D) R; dJames L. Hartley
. }" D8 ?8 C% M  W" p5 [. N  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13
/ S9 B* g7 f/ l* VLucia Baldi, David L. Hacker, Carine Meerschman,
5 E8 S( [3 @$ G0 _$ Land Florian M. Wurm- ^1 {4 g# J9 p
  3  Selection of High Expressing Mammalian Cells by Surface Display / b" j0 Z& w1 w; f+ x
of Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27
3 m4 g  V/ G! @3 s4 g1 H  LChristine T. DeMaria, P( i/ r3 Y5 a% q6 u
  4  Expression of a Secreted Protein in Mammalian Cells Using
$ u6 n( j8 ~$ O) f- f$ T1 |3 X/ gBaculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41% U1 P7 ^* D5 L; t
Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash. p1 G. d/ `- }' M3 L
  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65
6 I' X: {. p2 Z) b" O0 ^' J9 }Oliver Gresch and Ludger Altrogge" Z" c6 T2 f7 B3 ]' _* }9 L
  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  75. e6 @3 C- T  P4 S
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, 1 n) I# a9 g1 A# I3 W( \
Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch
7 _. W8 M( `8 [* y2 ~$ s- c  7  Using Matrix Attachment Regions to Improve Recombinant
, ]- x& d$ E/ @0 Y/ ~- UProtein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  93
& ~3 h, N2 P+ ?8 Y3 U  WNiamh Harraghy, Montserrat Buceta, Alexandre Regamey, . I4 ]5 O5 N# V7 L6 Q! ~
Pierre-Alain Girod, and Nicolas Mermod
1 z! H7 C8 f: i# h# N' N7 I5 o9 Z  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111( h; a0 E2 q8 v8 G/ W+ v
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh,
/ o; p0 b3 w7 s7 n( Zand Tina M. Sauerwald
3 ?1 j0 b5 h% P8 M7 B" {0 q  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene 8 X4 H2 f. R. ^" J. ]% T1 x& P6 M
Expression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  125
3 X0 w" `( X/ L0 \5 ?6 {, dMariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang# e- h, Q6 ]; a, N; \& W
10  Converting Monoclonal Antibodies into Fab Fragments for Transient & x/ p8 t( e' w
Expression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
9 z: V2 C5 B$ l  h* |1 DJoanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq,
; a5 G, c8 y  P; n) t. sRebecca Hamer, and Raymond J. Owens
6 Q+ {  }& _3 z/ A  R5 V11  Generation of High-Expressing Cells by Methotrexate Amplification
8 A( F9 a( `2 i7 X6 s- Y2 a6 Lof Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  161
; C/ e; E) b- J1 qSay Kong Ng4 x% c' m% d) y6 X4 K, `) d
12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  173
+ p) N4 R( C3 j" h6 M* [Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia
. W- ~- C0 x; S! s: J3 {4 Q( Zviii Contents
$ G- _$ F4 h& f2 L* i. G13  Identification and Characterization of Protein Glycosylation Using Specific
4 g, L) w. E) K; Z. cEndo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189" A3 H" b" _3 t; `6 j) i9 K8 l
Paula Magnelli, Alicia Bielik, and Ellen Guthrie. W& H: @, Q" B- k& M
14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213
0 r1 @0 K' K3 VRenate Kunert and Karola Vorauer-Uhl
  j0 ]* L5 o% d4 x15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  227# x1 {$ T: [$ m. n; Z9 V) M
Takefumi Sone and Fumio Imamoto
7 m1 b- t9 A8 O8 y16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  251, ]* e9 L6 S- H  }9 o/ _
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito# ?' Z4 S& j7 u# c( v! C
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269
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mimimi2256

沙发啊 很好的资料 谢谢楼主！

aidiulgy9517

好东东，谢分享

zorro

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daviddow

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ppcl2

Why Proteins in Mammalian Cells

dulaiyouyu

看看

cc1986

好东西

shixibao

lwqcd

good good book