PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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METHODS   IN  MOLECULAR  BIOLOGY4 W4 v  v0 U: x3 Q2 r  J
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Protein Expression in Mammalian Cells$ T3 m/ q* C$ u2 S( U. U1 y

+ n: \; q2 }2 c) T/ I$ f. R/ v  y- C" ]Methods and Protocols& x1 g2 y9 w* j9 P  c2 `  l

2 f1 V/ U2 v3 U+ u: L' i4 n5 O! HEdited by
  |# v- A- k3 U6 o/ |& |1 d% T: @James L. Hartley- k( W8 n* q3 m
Protein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute,
& z  |! A2 {' Q9 d3 D, \Frederick, MD, USA
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+ N6 X& z3 n( H! o- \( n/ NSpringer Science+Business Media, LLC 2012/ c- g9 i5 D) p7 L8 M" H
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Contents
& f- F# q  E3 pContents
  ~! ^; _* C2 [  M; YPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v2 j: G/ t: o8 f3 v& j
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix; c( K$ {" U+ @) w% ]
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
/ L4 f) i6 i8 P0 U' OJames L. Hartley
" Z( a" X" d( N( g/ S  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13
" U2 n" t5 O+ J  ]9 TLucia Baldi, David L. Hacker, Carine Meerschman,
/ e8 `& A) `. i9 V" a) g7 nand Florian M. Wurm; P  k# v. H& B2 d" J2 o& k9 g4 a% l$ u
  3  Selection of High Expressing Mammalian Cells by Surface Display 8 y2 S8 @2 `1 P6 Z4 z- q3 f4 Z8 M
of Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  278 g3 K' v9 a/ i6 f9 p( c
Christine T. DeMaria
( f8 y# ]7 b: C! [  4  Expression of a Secreted Protein in Mammalian Cells Using
! e- |% p  \7 o( w/ i( Z' fBaculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41
0 g/ s, y  V5 H$ Z8 r1 l$ eBarbara Ann Jardin, Cynthia B. Elias, and Satya Prakash
- _% G5 B& ^+ N6 u  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65/ O3 H: I  X' e9 @( i% n: b
Oliver Gresch and Ludger Altrogge
$ c  _% ^( J& {; }5 f* f; }  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  750 K' C( F0 W4 q( f5 S* G
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, # s( b! J, O) q' d3 P
Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch0 f3 x  K) G6 q
  7  Using Matrix Attachment Regions to Improve Recombinant 9 x- y" y+ T9 s8 \  k7 C- }+ t; `
Protein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  93
8 \( G% C1 T) Q. z7 }Niamh Harraghy, Montserrat Buceta, Alexandre Regamey,
/ a$ t; p- w( S% E% C+ [$ |; |1 PPierre-Alain Girod, and Nicolas Mermod2 s' y: H5 l" p5 g8 o& b# k; s) Q
  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111
. v7 a" b9 Z3 J1 @% wMatthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, , v7 h% E+ N; i
and Tina M. Sauerwald: U8 l9 {7 [) _
  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene
; [% }7 {$ v1 o) ^! C0 u& kExpression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  125' Q+ ~$ ^( Q! p8 r2 r4 S  {; x
Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
. y% N. L4 y& P- G, o10  Converting Monoclonal Antibodies into Fab Fragments for Transient
( [& c' x1 l3 ?Expression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137' \. u# P: X; y2 _! G
Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq,
& e, `5 S# x( ~1 ?# ^, LRebecca Hamer, and Raymond J. Owens
8 p# b  K6 m8 |+ |% O5 J* e5 M+ k11  Generation of High-Expressing Cells by Methotrexate Amplification 9 p$ ]; k6 h; `) n9 N% f
of Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  161
, O& x3 G5 Y0 ySay Kong Ng; a! h# e$ M% V1 b/ L# n" d
12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  173$ P* q: o9 [3 h, [7 @
Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia
- J! u9 R9 b! S$ x: w  hviii Contents
9 }; d5 F0 G" u" P5 o* y1 Z13  Identification and Characterization of Protein Glycosylation Using Specific - O& D8 y5 \" f1 b& I0 N  P8 q& [
Endo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189$ L/ p: _! n% Y
Paula Magnelli, Alicia Bielik, and Ellen Guthrie- J$ G- m6 d# J/ J- Q4 U
14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213
* x8 p  e5 m& g: ~: }+ B" {Renate Kunert and Karola Vorauer-Uhl
4 q7 k& q' t3 I( b- i15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  227" M) t  L3 p  G' r5 x4 _4 `6 W
Takefumi Sone and Fumio Imamoto
2 i" U, K. @) f16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  251
( r4 B2 U  D# P6 }" c; ^Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito
2 X7 h/ G7 y9 ?2 s9 AIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269! N! `0 Q) b; n& i+ d+ R
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mimimi2256

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Why Proteins in Mammalian Cells

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