StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子
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StemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。8 A6 ]8 q' ]! l
http://products.invitrogen.com/ivgn/product/10639011$ @. _4 u5 `, g+ b8 [/ t
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$ K8 v' D, r2 a  v至于STO feeders， 请看相关文献的描述：
7 ^* f7 p- g: M% [2 D3 C1 bSeveral alternative cell lines have been investigated  G6 F) g3 w& w6 B8 H+ @
for their ability to support existing hESC lines as well
# g4 A( r( O4 I+ J# u; tas being used to derive new hESC lines (Table 1). The
. D2 ?  @8 e9 \4 nmouse embryonic fibroblast cell line,STO, has been used
$ O  D. h; ?7 O  z, c( vto establish nine cell lines from frozen blastocysts and9 a9 g/ v, O: h3 Q; C& t
zygotes (Park et al., 2004). The advantage of STO cells
9 h' l4 q6 K! L6 `: C& A: F( uover primary cultures of MEFs is that, being immortalized,
2 O/ z' A. ?5 pthey are easy to maintain and propagate. Human( T& e8 T# Q% i7 ?
ES cells cultured on this feeder layer exhibited a similar. g# j* U  L5 _0 i' S! C
doubling time to those on MEFs and expressed surface
; d- \0 ~$ Q' k; Ymarkers as expected. In addition, prolonged culture did+ h7 w- a" ?" E2 N8 a5 j% q
not lead to abnormal karyotypes. However, Xu et al.
$ E1 H' V9 B% P  |( f3 k(2001) have found increased differentiation when conditioned& G- v# _4 v- l, ^! o
medium (CM) from these cells was used in
; |: @- Y9 X' bfeeder-free conditions. Thus, the STO line is not a direct8 I" w: r$ o* B+ b$ l
substitute for MEFs but can reduce some of the work
" b8 ~7 L" n( D! S/ ]; e1 K: hload regarding MEF isolation and culture.

yangpan521

非常感谢  受教了