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回复 yangpan521 的帖子5 q) Y5 V+ s! y6 V8 h. h
( G6 [9 x6 }2 ]StemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。: n" b4 M- [$ d# z
http://products.invitrogen.com/ivgn/product/106390119 V6 k) L' k4 G1 D: R# D7 ]. k' l/ v
0 c `- x5 h5 C5 Q! e: ^( c& m5 d: g- D2 J) c4 w$ B& O$ Z
至于STO feeders， 请看相关文献的描述： , A, f/ y: Q2 R) q8 Y
Several alternative cell lines have been investigated: E/ g1 e$ I0 R
for their ability to support existing hESC lines as well/ H4 }# w' s, \, }5 K
as being used to derive new hESC lines (Table 1). The
6 r0 @& t8 R4 D2 p# Pmouse embryonic fibroblast cell line,STO, has been used
5 T* z |! B$ x+ ^to establish nine cell lines from frozen blastocysts and
) l* O* i, n; V, Dzygotes (Park et al., 2004). The advantage of STO cells
& R3 V8 l( X& Y, B+ aover primary cultures of MEFs is that, being immortalized,; K o3 U* o+ L( G6 Y
they are easy to maintain and propagate. Human+ u8 T8 c$ E( U& U* k
ES cells cultured on this feeder layer exhibited a similar* G* e! \& L% n- ^- A7 n1 ?
doubling time to those on MEFs and expressed surface
8 A! [$ f# E i; f+ S4 _5 s) P6 W/ T# Smarkers as expected. In addition, prolonged culture did% R1 N! ]; @; m: n( [/ z% A+ [
not lead to abnormal karyotypes. However, Xu et al.$ L7 F/ d* Y3 F7 C, g0 V
(2001) have found increased differentiation when conditioned
* E6 ^& i+ N& q4 M! e$ {8 pmedium (CM) from these cells was used in
2 c7 s4 s4 P2 C/ I* p7 yfeeder-free conditions. Thus, the STO line is not a direct5 J% @9 M- N+ N9 _- R
substitute for MEFs but can reduce some of the work
' x5 V5 Y9 L+ Sload regarding MEF isolation and culture. |
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发表于 2012-2-10 21:43
发表于 2012-2-11 04:58
