大家好，我想培养有核红细胞，请教！！！

yaolinzhang

不知用gibco的STEMPRO-34SFM Complete Medium还是stem cell 的SFEM好？

xieyuhuan

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# t: ?* Q/ l0 B& i9 j" L你好，我想问下你是怎么培养的有核红细胞，我现在也想做这块，求指导，谢谢！

yaolinzhang

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之前计划做，但最终没有做。不过我可以给你提供几篇文献题目（我没有原文）：
) X% S# ]0 N6 b0 ], f# @5 l. q% R! @1.Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells& y6 f8 C' _7 P* \
2.Erythroid progenitor cells expanded from peripheral blood without mobilization or preselection: molecular characteristics and functional competence: R; T# f  L, ~+ x9 O' l  h
3.Red blood cell production from immortalized progenitor cell line
( x- C) F' s! Q1 {- a4 F4.In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems
& r) x" x; P& J5.Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion?; S% \2 F+ g( v# O* U5 A

xieyuhuan

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7 ]. Q% t" q1 x0 \有些文献我看了，就是每个实验室的条件不一样，所用的方法也不一样，目前正在尝试用EB球法做谢谢！