大家好，我想培养有核红细胞，请教！！！

yaolinzhang

不知用gibco的STEMPRO-34SFM Complete Medium还是stem cell 的SFEM好？

xieyuhuan

回复 yaolinzhang 的帖子! C) V+ ~3 A8 o

7 i. P  F% t. t9 @# A你好，我想问下你是怎么培养的有核红细胞，我现在也想做这块，求指导，谢谢！

yaolinzhang

回复 xieyuhuan 的帖子* K9 A0 N, ~! S" F: u
: M4 C6 e# q: h* u$ A
之前计划做，但最终没有做。不过我可以给你提供几篇文献题目（我没有原文）：
- ?! _- O) _. d% G- D2 J+ _1.Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells% v) m( {9 \* o* J
2.Erythroid progenitor cells expanded from peripheral blood without mobilization or preselection: molecular characteristics and functional competence
3 I+ x) H* p. h7 k- k3.Red blood cell production from immortalized progenitor cell line$ k7 M6 X: T+ m; F" O
4.In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems
# D3 R% `6 q: `+ j5.Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion?0 S5 x$ k6 r+ w6 M' O) K% v/ X, a

xieyuhuan

回复 yaolinzhang 的帖子, ^6 ^4 l& h: i: a& F& W9 g

" ~1 @5 O- N) C# }( h; @3 d; m有些文献我看了，就是每个实验室的条件不一样，所用的方法也不一样，目前正在尝试用EB球法做谢谢！