人iPS细胞成功诱导为功能性间充质干细胞

amywhere

香港大学Qilzhou Lian等的最新文章"Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice"中宣称：& T; b5 X- f& w$ ~! p

( L/ \% l) M, u, ^1 U第一次将人iPS细胞成功诱导为功能性间充质干细胞，将iPSC-MSC种植到小鼠身上发现可以明显改善小鼠后肢严重缺血并促进血管和肌肉再生。
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3 P" x* M3 r7 C& [8 e( DBackground—Aging and aging-related disorders impair the survival and differentiation potential of bone marrow
' f% F; Z6 d# H  ]mesenchymal stem cells (MSCs) and limit their therapeutic efficacy. Induced pluripotent stem cells (iPSCs) may provide0 a) e/ @% |/ T" n/ t5 ]& G; f
an alternative source of functional MSCs for tissue repair. This study aimed to generate and characterize human+ m& a$ Z; H( G- ^9 _
iPSC-derived MSCs and to investigate their biological function for the treatment of limb ischemia.
2 ~( r7 E  |; H" V$ GMethods and Results—Human iPSCs were induced to MSC differentiation with a clinically compliant protocol. Three
2 T1 [# v  W6 i5 ^. i3 y) Qmonoclonal, karyotypically stable, and functional MSC-like cultures were successfully isolated using a combination of
$ y5 h  U3 c1 `7 \% d; l9 ~1 ]) lCD24
and CD105 sorting. They did not express pluripotent-associated markers but displayed MSC surface antigens! ~, [9 t7 \0 h# w8 w" ~/ \7 N: C" z) b
and differentiated into adipocytes, osteocytes, and chondrocytes. Transplanting iPSC-MSCs into mice significantly7 I0 Z/ _' b7 a5 h2 \$ p/ x
attenuated severe hind-limb ischemia and promoted vascular and muscle regeneration. The benefits of iPSC-MSCs on. k0 P, l+ \* ]3 a- ~# s
limb ischemia were superior to those of adult bone marrow MSCs. The greater potential of iPSC-MSCs may be
# g! K  c( V8 K, U1 Vattributable to their superior survival and engraftment after transplantation to induce vascular and muscle regeneration6 C8 G7 d% q9 g6 c% Y! B% _; K) F* E
via direct de novo differentiation and paracrine mechanisms.# S: _6 [' r5 j
Conclusions—Functional MSCs can be clonally generated, beginning at a single-cell level, from human iPSCs./ D. r$ a; Y) Y7 s7 x
Patient-specific iPSC-MSCs can be prepared as an “off-the-shelf” format for the treatment of tissue ischemia./ L" n' o% Q$ W
(Circulation. 2010;121:1113-1123.)
1 i/ R9 X; Z0 s. k, kKey Words: induced pluripotent stem cells
ischemia
mesenchymal stem cells
peripheral vascular disease
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repair
transplantation

amywhere

2010年3月的，有些老了的文。最近正在整理相关资料，看这篇不错，论坛上没有，就拿上来分享了。