Te Trophectoderm. The outer epithelial layer of the blastocyst% H. N" ]. x' d' L
consisting of polar and mural components that line the ICM and& ]2 y' T5 t6 q( C) k
blastocoel, respectively. 滋养外胚层
我不知道你的意思是什么 你之前引用的文章明显和你上面表达的意思不一致 及何为all ips小鼠" F# O+ ~5 g! p( j0 B6 u
Such a tetraploid embryo can develop normally to the blastocyst stage and will implant in the wall of the uterus. The tetraploid cells can form the extra-embryonic tissue (placenta etc.), however a proper fetus will rarely develop 6 {3 m1 s/ j+ Q' q/ V0 Z ; r' M# Z. w0 H/ s0 K @7 X u这句话说的是tetraploid cells可以形成胚胎外组织 但是他们发育到囊胚就不能发育下去了 而小鼠是由te以外的细胞形成的 你之前的认识明显有误 4 i- h& c2 q. g$ v7 b" Y可以看看四倍体补偿相关的文章和原理
Tetraploid complementation assay * U) ?. E- Z4 W* \; w
http://en.wikipedia.org/wiki/Tetraploid_complementation_assay ( i7 V. H& M/ q) R" T" A9 O8 y. D3 fNormal mammalian somatic cells are diploid: each chromosome (and thus every gene) is present in duplicate. The assay starts with producing a tetraploid cell in which every chromosome exists fourfold. This is done by taking an embryo at the two-cell stage and fusing the two cells by applying an electrical current. The resulting tetraploid cell will continue to divide, and all daughter cells will also be tetraploid.6 \* J" I& i$ {* l& g: `0 z5 _
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Such a tetraploid embryo can develop normally to the blastocyst stage and will implant in the wall of the uterus. The tetraploid cells can form the extra-embryonic tissue (placenta etc.), however a proper fetus will rarely develop. 0 W, L' D. w. \! F1 v5 N( a5 ]+ P p
这是我的理解，请抹了个茶指正。
是可以的，对于小鼠的是可以了，对于小鼠IPS的全能性的鉴定的是tetraploid complementation.这个也发表文章啦，但是对于hiPS就没法验证，只能做畸胎瘤。iPS cells produce viable mice through tetraploid complementation.' m) D; C6 v" y- x. V0 n) Z+ G
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F, Zhou Q.0 i+ f/ \8 f- j2 D* |+ _( j
SourceState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. ) Q' z/ H; v- M u. ^! ~2 k4 I
发表于 2012-3-30 09:17
发表于 2012-4-6 21:36
