是可以的，对于小鼠的是可以了，对于小鼠IPS的全能性的鉴定的是tetraploid complementation.这个也发表文章啦，但是对于hiPS就没法验证，只能做畸胎瘤。iPS cells produce viable mice through tetraploid complementation.8 \2 `1 l8 }1 l/ K/ E" F
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F, Zhou Q./ H* x4 Y; ]" f
SourceState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.- M. h2 K4 O1 `7 j" L* ^4 Q: V
Tetraploid complementation assay 3 u& b7 m9 ?3 K) `3 S; Zhttp://en.wikipedia.org/wiki/Tetraploid_complementation_assay6 G% C# V" [+ X
Normal mammalian somatic cells are diploid: each chromosome (and thus every gene) is present in duplicate. The assay starts with producing a tetraploid cell in which every chromosome exists fourfold. This is done by taking an embryo at the two-cell stage and fusing the two cells by applying an electrical current. The resulting tetraploid cell will continue to divide, and all daughter cells will also be tetraploid. ) J7 j6 A% ?! |/ ? - U* t- A# V& H. t8 m. tSuch a tetraploid embryo can develop normally to the blastocyst stage and will implant in the wall of the uterus. The tetraploid cells can form the extra-embryonic tissue (placenta etc.), however a proper fetus will rarely develop.# }! \- C- ?3 T
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这是我的理解，请抹了个茶指正。
我不知道你的意思是什么 你之前引用的文章明显和你上面表达的意思不一致 及何为all ips小鼠/ Q, |; f8 [+ a3 }7 W: T- L
Such a tetraploid embryo can develop normally to the blastocyst stage and will implant in the wall of the uterus. The tetraploid cells can form the extra-embryonic tissue (placenta etc.), however a proper fetus will rarely develop: \$ g* |: o- L% O7 l2 t
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这句话说的是tetraploid cells可以形成胚胎外组织 但是他们发育到囊胚就不能发育下去了 而小鼠是由te以外的细胞形成的 你之前的认识明显有误 + U7 l+ B) a! T! z4 ^0 [可以看看四倍体补偿相关的文章和原理
Te Trophectoderm. The outer epithelial layer of the blastocyst' E. Q# C2 }2 g8 x
consisting of polar and mural components that line the ICM and8 y! D d ~/ ^2 U
blastocoel, respectively. 滋养外胚层
发表于 2012-3-30 09:17
发表于 2012-3-31 10:30
