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1 }+ Y$ j2 E2 E  T" @6 A& oNature Biotechnology 14, 185 - 188 (1996)
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- M- r: _% L4 M; vImproved Wool Production in Transgenic Sheep Expressing Insulin-like Growth Factor 1  h# v" _1 m+ ]' _$ |) w. v
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Sami Damak1, Hung-yi Su1, ,*, Nigel P. Jay2 & David W. Bullock1, ,2, ,*
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Abstract
- L, s* s7 u* J( ]& \( LTransgenic sheep were produced by pronuclear microinjection with a mouse ultra-high-sulfur keratin promoter linked to an ovine insulin-like growth factor 1 (IGF1) cDNA. Five transgenic lambs resulted from the microinjection of 591 embryos; one male and one female showed IGF1 expression in the skin. A progeny test of the ram was carried out by matings to 43 non-transgenic ewes. Of 85 lambs born, 43 (50.6%) were transgenic. At yearling shearing (approximately 14 months of age), clean fleece weight was on average 6.2% greater in transgenic animals than in their non-transgenic half-sibs, with a greater effect in males (9.2%) than females (3.4%). Transgenics showed a small but significant increase in bulk, but male transgenics had a lower staple strength than female transgenics and non-transgenics which did not differ significantly. There were no significant differences in fiber diameter, medullation, and hogget body weight. To our knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction.
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http://www.nature.com/nbt/journal/v14/n2/pdf/nbt0296-185.pdf

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