PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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Springer MMB Vol931 2013 共29章 552pages- C$ C  V" G8 Y
Cell Imaging Techniques  Methods and Protocols4 y' q1 ]+ }0 [' ^9 b" b+ D
Second Edition* K6 h4 `+ d& P9 a# s$ z6 r
Edited by
$ h6 v" D; N4 u% {Douglas J. Taatjes) _# G# ?1 v8 F( _) l
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Contents9 \; l2 r# h( [* s, c0 I

$ ?& k5 O) @0 C5 @9 k+ V! oPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
( S/ A' u- o& CContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii7 }9 \  j9 m; s+ o  I# k" G" b/ W8 Y
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       14 N% h4 y$ C) N/ o5 Y1 k- M$ W
Douglas W. Cromey1 Z2 t( |8 R& G
2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29
2 A$ I& I6 M$ C* TDonna J. Webb and Claire M. Brown* B& a3 c- b% ?1 K: V
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61
0 z* U' O# S9 ?3 D* ?. S' jJ. Lacoste, K. Young, and Claire M. Brown
. W7 {5 Y& S6 A4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic 7 Q0 n( Y. T$ ^
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
( W& r, ?2 v. Z+ R  f. `Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
) ^, E2 ]6 c6 g% K5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97% K7 @( H) b! d( L
Jeremy Adler and Ingela Parmryd
* a/ [" t" m. X0 C' Z: B6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111* L* F* O5 ^7 |! _5 Q5 K# R
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman" B4 I4 z2 S0 B, z
7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123' }  F, |' |0 T+ z9 B) w
Raju Tomer, Khaled Khairy, and Philipp J. Keller0 s( w! ?! T) j
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139. R. M' _' X8 C0 ~7 _3 m
Lasse Evensen, Wolfgang Link, and James B. Lorens& l( {6 T" O4 q# c" l, x# g/ N" [# A
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153
8 a) ^& [/ w' D: UAndrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
+ E+ h7 e9 g: }  u10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential ; c% j: e- m/ `  D& G
Interference/ x: L9 |1 V9 T, r! l
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169
3 K& _- y. S" @% j2 z9 ?, CWei Sun, Lehui Xiao, and Ning Fang
2 h1 {1 L, j5 g( g11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187; {9 r' e- {/ P( J# i
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz2 E1 l& s8 p: T/ C
12   Laser Capture Microdissection for Protein& ?5 ?2 l2 |% t% E: |
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213& Q& k5 [$ V/ @/ a' B( N5 I+ L
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,/ W1 K7 H+ f. G+ T8 o' V
Lance A. Liotta, and Virginia Espina- }) W0 V8 F3 C$ n" K3 e
13   Viewing Dynamic Interactions of Proteins and a Model Lipid
& N; [& n+ ~* S. XMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
" ~) P! o6 m/ \2 Q8 AAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes6 h2 Q0 c, x! V* o7 u% S/ \
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
* r: n: H% g6 _3 ^1 B1 o" mLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko: |0 m3 J+ P+ ~% a' q
15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313, g7 I. \) L7 ^* L" T6 u4 Q0 F
Joanna L. MacKay and Sanjay Kumar
9 [$ j; t3 o2 S7 }% G' a4 M- b16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
2 I- F- B; Y/ J2 |1 Z7 ^& b( `3 CLilia A. Chtcheglova and Peter Hinterdorfer
+ s5 b5 P9 H: T9 a17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345
9 y4 w3 q" s1 HBhanu P. Jena& U$ Y1 n( G& R; C$ J$ H2 |
18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367" ~/ @0 g/ D9 o# v1 Q
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
) N. w9 i% Y6 y! h$ A" ]" D# {+ n19 A Novel Combined Imaging/Morphometrical Method for the Analysis; @7 x8 F0 s2 u2 L; S- \
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391
/ r6 a& N6 y# o; u1 iMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
+ r0 Z% U7 @3 r( U" p% @20   Correlative Light–Electron Microscopy as a Tool to Study: f1 `! d" E: J6 @+ O. w" Y
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413
! A; C$ b& M. sElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini4 X5 T; C( @( l" k
21   Photooxidation Technology for Correlative Light
/ p+ Z$ r: y- g% a! Cand Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423; f3 w9 b- h, k* u" L
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,   R' @; C9 T) d+ x) `* x
Margit Pavelka, and Adolf Ellinger
7 V( D7 {1 s8 g2 \9 |8 y, y4 v& S22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437' V7 P, {# Z  l' }" Q: y
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and & R% ?! o6 Y- z8 [, [9 j5 b
Margit Pavelka
! F/ c& X7 q: K23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449: k. R/ T6 c, [* W6 D. K
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
$ X4 r4 ?. q) W# B0 L* @  ^5 ^24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
9 l! V5 _! z, x( W0 sNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader% z5 Q4 v6 G" r/ U
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483: P; B' x+ V* j  S$ s
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
  B( `) L6 q  H26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493
: Y8 x! e8 P5 }0 v8 IJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
  n! u! d: p, I27   Environmental Scanning Electron Microscopy Gold
3 K" U/ Y( @! @, g+ g! k1 zImmunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517! u+ z, i( v. e& l5 B
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi; K5 _! U: [' ~: g* m  ^0 c) h
28   High-Pressure Freezing for Scanning Transmission Electron7 c/ ~- g1 t$ b5 a' m' U
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525' \! ^- j& S  L7 q+ _
Paul Walther, Eberhard Schmid, and Katharina Höhn
. T) Q8 P6 ~5 a2 B* \8 a3 E29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537
' m$ m1 Y. e" o$ \/ H9 wStephan Meding and Axel Walch
7 \; S$ ^, U! u0 MIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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