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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 - n! W* V# c, @% v" G
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& S" j; M0 E4 J) A8 ^Springer MMB Vol931 2013 共29章 552pages
7 E5 M; N1 y- @9 [Cell Imaging Techniques Methods and Protocols1 K/ Z) J! }& w8 e. U8 h% j( y4 s: \
Second Edition! ^" r3 n4 \* _* k, _9 R; ]
Edited by
9 r) z) q& r' Y* M/ r9 g7 fDouglas J. Taatjes
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1 u3 T6 t* B) i4 Z( D- C$ C- K/ ~% |, m4 Y9 k+ {# R% `
Contents5 z$ Y; R z- A' |
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
) b" R5 Z- v1 B/ B4 g$ TContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii T3 U4 S+ s( @8 n
1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 1* `' n/ `" e( S
Douglas W. Cromey
; }2 `. _/ J% d2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
( ]2 Q k. O- J1 ^3 cDonna J. Webb and Claire M. Brown2 [6 p, v( h$ p: h2 [* T2 e0 V. w
3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 61
* H* ^$ H, c$ ^% M- qJ. Lacoste, K. Young, and Claire M. Brown; {. X1 t5 D3 N' L3 T! G1 C. m
4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
# _9 g5 Z7 ?. e9 P3 l& UProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85" L8 a5 ?9 P( T
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
" x4 u; R, M4 O6 z5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97
% t) G" l5 S2 KJeremy Adler and Ingela Parmryd
' s) B9 t+ a, Y7 _' @) j6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 111
+ E$ i( r% z2 d" h1 \Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
, i, _* ~& L2 U* J7 M3 L7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123. m- X8 z; x% z; o
Raju Tomer, Khaled Khairy, and Philipp J. Keller
- w: Z- e l9 d8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
1 R7 V+ n. u* d# Q# @Lasse Evensen, Wolfgang Link, and James B. Lorens8 Z7 W" J. d- v* X5 T* X
9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153
- a, W; g3 B/ b; ?$ SAndrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
! h5 S2 b9 F; e* b# @5 }10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
- j- g; ]! g2 |0 J! U2 s1 @0 `Interference$ P$ K& i( I1 |: Q4 X7 d& c6 ]8 P
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 1696 D: m0 i- R" y+ ~
Wei Sun, Lehui Xiao, and Ning Fang* I. F* Q* v/ i" [, ?, O' w/ D
11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 187/ H; D) Q$ S. v$ w
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz2 Q r# V7 q) ]4 f+ K9 d9 M
12 Laser Capture Microdissection for Protein* b- A$ r5 ~, H- U- s
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
& a: N& X& A: p9 y% iYelena Golubeva, Rosalba Salcedo, Claudius Mueller,
, C0 W+ {$ X" m, RLance A. Liotta, and Virginia Espina
2 c7 X- A% J' y, C13 Viewing Dynamic Interactions of Proteins and a Model Lipid8 U0 ?# g c8 n6 @
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
( E! D4 X5 O7 J lAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes: K. N# R! c! E4 d, H
14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295! u% i+ E% X5 d1 I9 q
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
( b; _: _$ V3 ~5 s: i15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
2 ?+ u) O+ _1 K0 ~Joanna L. MacKay and Sanjay Kumar
# C2 K" M8 x4 O5 @) T/ u- H) D6 {16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
8 N% p& N2 M5 j: ~Lilia A. Chtcheglova and Peter Hinterdorfer
, S, R& \9 l) A5 p4 t8 _5 r3 ]17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 345
5 Z8 u! h. q, f$ a# A, L8 C6 i& qBhanu P. Jena/ P, [! X# {+ ?( H* g- t
18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 367$ S9 A2 p0 ~2 f0 q Q( s
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs& D9 Q9 C4 }0 C, G
19 A Novel Combined Imaging/Morphometrical Method for the Analysis
8 S+ P8 |$ M" l5 Kof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 391+ R: }+ K+ i0 q i s
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes; O5 c) {7 ^: R
20 Correlative Light–Electron Microscopy as a Tool to Study
: d+ {$ T( B6 P4 u9 y9 eIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 4135 a6 K2 `- Y3 {1 I, A+ C# {
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
: f& m5 j" R5 }+ M21 Photooxidation Technology for Correlative Light
\+ V: d7 v/ h x; ^$ b. V1 ^# hand Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423. k0 O5 B) A. u
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 8 M0 U6 `* n9 c! R
Margit Pavelka, and Adolf Ellinger4 f) _1 d' F* |- k! k% h7 @7 s e
22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 4372 {" N7 o* g% C s: ^8 O/ V. g* b
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
3 P" K3 B9 L9 o( mMargit Pavelka
* P3 n% }$ l: s5 F' Q3 n7 I f23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 449! r% M; ]8 f" @; q
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
/ M4 m+ u; L- V+ \; i* P$ X8 M24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 467; \8 t; I1 L' X1 j5 L, u, v$ v
Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
# T2 f* D/ B) R4 |25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 483
# E: b$ X" x1 {# O# ]" DMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto+ b& ~; Z: S: v; y8 H- D' k% y; b
26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493( e( \" }& I0 X- V
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
7 z) _/ r( V$ Q" m, ~6 h, L3 B4 F27 Environmental Scanning Electron Microscopy Gold
3 X. R* o4 m' D( s- X6 E2 [( K) BImmunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5174 m8 l6 W& a- A* a# m0 N
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi- k1 K! x% k( w- W! V6 u% b* N1 z; W
28 High-Pressure Freezing for Scanning Transmission Electron
& x8 I. B5 o1 C8 N" t8 O/ p& i7 MTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 525+ Z; Y6 M: g. M- U: h M0 I4 e% _
Paul Walther, Eberhard Schmid, and Katharina Höhn4 y$ b( U: }# s! g2 R* ^
29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 537" Q& ~+ r" }/ \3 ], E
Stephan Meding and Axel Walch6 s" f" o' `$ {; R
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547& ^6 |! V) C5 R' j( r9 H7 V0 J
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