PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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Springer MMB Vol931 2013 共29章 552pages
& [  b0 c% D# e2 cCell Imaging Techniques  Methods and Protocols0 ~8 q1 A% K: ]( ~6 s* N7 U- {3 n
Second Edition
* j) f# @9 K2 n  A5 I! [Edited by2 s. h2 E! G- h/ E: l3 e
Douglas J. Taatjes
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Contents
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3 j8 I& b  V* R0 SPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii* L) v/ C, t/ l2 L. g5 V% v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii! p; j5 c# d3 W! j' L1 j
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
9 n9 F  W: R9 a# X. l- wDouglas W. Cromey
8 U) k; z# W( c8 I5 J) F, N% d2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29
. }, [/ L: }0 g% S% IDonna J. Webb and Claire M. Brown" S( ]4 b4 Z+ R5 b$ r5 p
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   614 Z. h& g3 Q" x' T' E  y% F2 D& F1 R/ j
J. Lacoste, K. Young, and Claire M. Brown
! v6 j1 C* w5 m) o$ X4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
' @+ Z  ]& [8 |. D* B! yProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
& W& Z) z9 Z7 X0 c8 G" \- DWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill4 }1 @) L0 q/ g) W1 j8 l# k
5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97/ b2 B, [5 t% M" H; K
Jeremy Adler and Ingela Parmryd& g$ ^# B8 M0 ~3 l0 b5 t$ H
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111
: l- L' U, v* }  H3 NSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman& ]. e% u6 R3 c; ^! z' w7 |* r
7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
) n5 t6 M# x" v9 _2 z  a3 uRaju Tomer, Khaled Khairy, and Philipp J. Keller
' Y5 y+ w* ^( {7 [8 b8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
' |; @# B; Q- \  s' D" Z; ELasse Evensen, Wolfgang Link, and James B. Lorens
3 _+ R4 V/ N  F! ]9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153+ \5 v2 U$ H% z9 C
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert+ f) ?3 Y5 N2 c
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
4 z4 M4 C$ O$ {Interference
7 @$ |% n" ?2 L% V5 K/ y, tContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169- F3 p3 N) q9 X0 c  O3 W
Wei Sun, Lehui Xiao, and Ning Fang6 x- Y1 g. h) p/ D/ N
11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     1876 Q/ [" P& z/ K6 N( g3 D
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
$ q$ Q8 B' D4 Y& z12   Laser Capture Microdissection for Protein; D! y8 o' X" h2 G8 e& l% p. k6 |; n3 ]
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
: v8 `/ X1 h4 TYelena Golubeva, Rosalba Salcedo, Claudius Mueller,9 O% t0 J3 L8 q, u  h& Z  H: o' [) I
Lance A. Liotta, and Virginia Espina
6 ?$ L3 F! }" p% X13   Viewing Dynamic Interactions of Proteins and a Model Lipid9 r. L! }8 \- V) P
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259. K- B% U$ C& ?( V0 y6 |  G
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes7 D2 b) q/ E4 L; z* ~, v, b
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .2951 Q5 a* n8 d9 M6 L/ K0 ~3 W
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko/ t, X  z9 d( `7 F5 G3 W. T6 E
15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
6 p, X4 @. w7 j7 E2 BJoanna L. MacKay and Sanjay Kumar
" k* C( ^3 U- `) Y16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
/ t" A, ^/ q4 e9 V& H% oLilia A. Chtcheglova and Peter Hinterdorfer3 x2 e8 b# x) j
17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345
- f( Q1 l( ]/ g! _. fBhanu P. Jena
* _9 Q1 B, e& Y2 g18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367
/ {" d% C& L+ ^7 iChristian Mühlfeld, Lars Knudsen, and Matthias Ochs# K( Y/ K& q$ Z+ R% o9 D1 n
19 A Novel Combined Imaging/Morphometrical Method for the Analysis; n* y1 l* Z/ C4 `3 H5 g3 N3 E* Z1 Z
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391
% p: F" ]- U6 s* u5 J" vMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes* g( `& n, K- x" d0 k. a
20   Correlative Light–Electron Microscopy as a Tool to Study
" O: c% s/ U8 x, ]. Y- {$ gIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    4132 j4 N! E7 F- h5 d  Z( g# j
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
% S" y( E% v: x5 V21   Photooxidation Technology for Correlative Light  D6 L( o& w- c8 M: i
and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423
& Q/ ~" O3 u' _# c2 ~Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 7 F( R( ^8 @6 x9 ?
Margit Pavelka, and Adolf Ellinger1 p* y# C9 l$ C" H- Z2 `
22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437
! Y) m- l. p# }+ NCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and # n8 o: G, t9 D+ b9 g& W
Margit Pavelka' Y, B. o1 a9 n3 K* m  C4 L" K7 I
23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449
4 t) C$ s  h6 h" N- w8 j2 p, kKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
* L3 }+ H* }4 R' \. l" U24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
. R! k! p' U' yNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
  J( i) q0 E' f4 Q- F% |/ F25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483
4 a- g. W4 {! \4 ~$ b' P# _8 x" \7 WMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto  l9 Z/ X" E4 S, X9 U
26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493" Y  q4 ~/ ], F5 R8 E. ?
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald/ u: |9 ]7 k2 \- c  t
27   Environmental Scanning Electron Microscopy Gold/ u9 n; l* L: K+ t7 |
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    5173 ?, I3 y' V0 ?$ V! O) L
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi/ X/ g- d4 B" ^- L' d) k
28   High-Pressure Freezing for Scanning Transmission Electron
6 m, Z6 _3 x. L! r  OTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525
% j6 D5 f8 L2 t+ d" B6 KPaul Walther, Eberhard Schmid, and Katharina Höhn
! \" \! ~2 A/ Y29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     5370 V6 f4 [% T; \/ V1 c
Stephan Meding and Axel Walch' T8 h3 z& y4 }0 ~8 _! \2 e/ W
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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Cell Imaging Techniques  Methods and Protocols
' t& s8 m' Z' k1 y7 c* R2 VSecond Edition

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