PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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; D4 \& o8 _9 w3 r& ASpringer MMB Vol931 2013 共29章 552pages  Z. `* b6 {* R( l0 q
Cell Imaging Techniques  Methods and Protocols) }6 V$ P7 w* J+ P
Second Edition$ O7 Z; w) _; Z  G( p
Edited by
% h5 C3 Z' D" F3 F; \5 l: KDouglas J. Taatjes
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- c- d5 G, \2 W0 m8 ]4 E0 |Contents
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
) I# k3 x2 u/ _( NContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii3 B6 A, Q6 @) V- v0 @
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
2 K2 T, N/ Y* P8 YDouglas W. Cromey
- N0 C( {  `8 Y1 F# ]0 S0 f' P  V2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29' W: t2 x/ _2 D& o, U+ f
Donna J. Webb and Claire M. Brown0 y3 C. E7 a, L
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61
1 v$ y' s7 v8 f5 [+ H% vJ. Lacoste, K. Young, and Claire M. Brown( F6 y0 q- b" B7 j. y2 E  E
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
7 R: g1 u/ d- ~, j% x- sProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
, b7 M. S. J: J: I& _Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
1 @8 r9 F$ [: M5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
) _" z. \6 [# V  [- B5 IJeremy Adler and Ingela Parmryd( t$ f1 @% U  [( I( Z
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    1116 _8 _, \7 d8 r$ f3 r0 y: X5 y
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
% b, |' V2 }, _; ^7 g* N! K7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123' e- O4 V' [$ D& L
Raju Tomer, Khaled Khairy, and Philipp J. Keller
" ~& c3 A( R, U: D8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139  U2 g: i' E* w2 C% E' U. q7 J& j% Z
Lasse Evensen, Wolfgang Link, and James B. Lorens2 _. f7 j* V4 t( I. H
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153. l: l* p* c4 a" ]9 h7 q3 h
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert. I3 V9 r+ |4 Z9 ^9 ^" {
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential ' [) N8 S' B2 N0 O5 Z5 ]9 A0 l
Interference
1 f# z1 X  p9 C) ~; lContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     1697 O" o5 j  b. P6 _* V, f; Q
Wei Sun, Lehui Xiao, and Ning Fang! i4 F& n) u) j8 y" @) t  F/ c
11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
. ?( b& \5 M  [) _% u1 F2 FPiotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz  z  Z3 u* Z$ b; O4 T. o% v7 w
12   Laser Capture Microdissection for Protein4 s6 E- W: U) C0 _: z: p8 A! @
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
4 z1 N9 [6 U2 @0 N* m7 g. _: DYelena Golubeva, Rosalba Salcedo, Claudius Mueller,* T) ~. m+ u( C& w: `% M9 {
Lance A. Liotta, and Virginia Espina5 p0 |9 D( W4 t4 {$ F
13   Viewing Dynamic Interactions of Proteins and a Model Lipid5 w( s0 Y" ?9 m
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
7 X5 t" ?5 d# K; HAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes1 P/ m$ G2 m( d' X+ P
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295/ U$ `8 T* k4 `. D& x
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
) s6 F2 z- c% h15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
  v" B8 n9 k  k. ]8 f8 tJoanna L. MacKay and Sanjay Kumar
4 P3 ~) g$ Y8 V  \1 w3 e# l" c) x16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
+ F: p: r/ D! @9 L; R+ z/ T1 v' i, gLilia A. Chtcheglova and Peter Hinterdorfer) ^0 A3 F$ }% f# q; ^8 V9 F% P
17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     3458 ?$ o  g1 L& y" M
Bhanu P. Jena! w( x4 w/ R3 N. M$ x! z
18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367
. a% E7 j& p. E3 G3 TChristian Mühlfeld, Lars Knudsen, and Matthias Ochs3 A, n' _$ k) z- g2 G9 p
19 A Novel Combined Imaging/Morphometrical Method for the Analysis/ y3 ~( C4 c, N3 o  q( K! ^5 k. W
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    3917 N7 }' w# X! K( F
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
) w% j5 f2 o% k8 m20   Correlative Light–Electron Microscopy as a Tool to Study
! n- ?# W  ~) w( {/ [4 hIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413
! a2 Q0 w# ]4 [! v5 r; G4 X% j5 kElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
* H  W! g6 K; D21   Photooxidation Technology for Correlative Light
! W* a+ W( P( s/ w( I- ^and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423  d) a( M8 q- H
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, " N9 m+ [6 u( O
Margit Pavelka, and Adolf Ellinger
! j& x6 ]6 T& M3 W" K9 `22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  4371 X7 G) u8 h- s9 Y: ?; q
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and - w3 _( k& s) Q1 f
Margit Pavelka/ A# Z& g! t/ X$ M" }  t
23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     4494 P0 ?6 ]* b0 ]$ |  l
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori# b/ v  A; q% @3 e! Z, q
24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467$ C9 y- J4 U) E7 x( P: v$ c6 y: r
Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader, _. i% P+ F$ X# l; `4 ~
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483. y) M9 f3 ?. G9 k# U0 X
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto, J0 e/ V9 [2 ^$ u4 g
26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    4935 a  i  a1 O3 P# _# h
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
$ O# U- I' R" w) J27   Environmental Scanning Electron Microscopy Gold
& H9 i; R5 ^8 l  `Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517- F& {% Y: e# M0 s$ Q% f( ?
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
( e! H+ U. Y' f! i28   High-Pressure Freezing for Scanning Transmission Electron
$ R7 E4 [% ]- |: {  YTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     5251 z0 o" r7 r* m( R
Paul Walther, Eberhard Schmid, and Katharina Höhn
  ?5 y$ q1 |& W. V" T: W29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     5376 N9 G2 y4 Y% O5 _" s0 ]  p
Stephan Meding and Axel Walch
7 V- a2 l& I# u/ R2 wIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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