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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 ' G. z; I: d+ Y1 [
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3 y; k% j; Y3 I* w I% ISpringer MMB Vol931 2013 共29章 552pages
( c' K! @( A0 T2 F, yCell Imaging Techniques Methods and Protocols
* }8 {4 k) e( M4 {( USecond Edition8 c4 l4 }$ W- N0 W- G+ y \
Edited by
* C a" N' [- x+ hDouglas J. Taatjes- n0 _) \7 t; E6 |2 t* Y
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) o* U) K& ^6 W% V$ DContents
/ |. V" V" [- T( N- [; y$ ^ I0 o# K( y& `
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii" D6 k- ~( d2 b
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
2 ?6 ]* c& { m: g6 C& I1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 14 o3 k ?4 x' n, r6 f/ D
Douglas W. Cromey3 n S+ ] o/ @1 A) G/ w
2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4 b; k8 E! N1 J1 E* D GDonna J. Webb and Claire M. Brown# z7 Y$ J( L0 z7 a
3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 61" R( K. o5 k, t5 r1 l
J. Lacoste, K. Young, and Claire M. Brown
8 \' I0 a. T8 x, A$ u4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic g' v/ }* N$ K( d9 y
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 856 ?; Z& z+ J" T* ~3 G
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
% ]5 C3 w2 P# s4 e% |5 {: d3 a5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97& e. m- ? K0 x: \7 f x* a
Jeremy Adler and Ingela Parmryd- Y" b6 \! h+ g2 i
6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 111$ L5 ^2 {" x# X1 `& l2 a5 y
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
* a6 h$ v/ u3 n4 X( _7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123. r7 e& w! y% X, i! m
Raju Tomer, Khaled Khairy, and Philipp J. Keller
! \! }; B/ Y; ^8 T8 ]/ b8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
% }% o1 } q/ dLasse Evensen, Wolfgang Link, and James B. Lorens; u1 P* X$ M7 d& H4 ]
9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153/ y( W+ k$ ^5 h! Y: o9 G
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert8 y& V( k! W, S" y+ f3 E
10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
$ R. W0 q A9 H6 A j$ `/ {Interference
8 C7 O* g* h/ d1 oContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 169
5 K. Z! [( u1 {- E Y3 [) |Wei Sun, Lehui Xiao, and Ning Fang
; f9 m9 F4 S# X$ I: o' p4 w n$ p! [11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 187# j. ^% K0 \$ f& R
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz! d/ m, g# R9 D2 i/ ^% W
12 Laser Capture Microdissection for Protein7 d- |' O6 ?7 d/ X6 e: J# X
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2133 b: O% M% a v' g& {! z! x' G
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
9 _5 o( k$ z( G' J6 ^# z, CLance A. Liotta, and Virginia Espina! q# S6 t& D5 Q% N4 Y: G5 E
13 Viewing Dynamic Interactions of Proteins and a Model Lipid
9 n2 ]" q. g& M; W3 oMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
( W. s+ L& s g- t% z7 P/ u" N9 lAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
' m: a8 D7 Z0 g; L. |4 D14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
+ a8 ?7 Z* ~. LLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
" M/ n/ q) Z1 ?( t15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313$ M, W3 {- d6 H* ?+ Y( u* Z- S
Joanna L. MacKay and Sanjay Kumar6 S9 l2 L( z8 ~4 s0 C9 ?
16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
( F4 W0 y7 h5 LLilia A. Chtcheglova and Peter Hinterdorfer ?" m: L1 q( Y2 K& o
17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 345
& {! V" t7 B1 sBhanu P. Jena A7 F) z0 a2 c; H3 N
18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 367 u+ l" K* p# C# k; s
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
/ h5 q5 Y( p0 L D. m+ X19 A Novel Combined Imaging/Morphometrical Method for the Analysis
2 j- \- W* A2 Q4 f8 r, {of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 391
8 t6 I$ K8 c, W% S8 Q! T' rMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
/ I+ R1 z; N, ]! W0 d20 Correlative Light–Electron Microscopy as a Tool to Study, v# m/ O& R: `& p, q" S
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 413+ F2 |9 b% }- H1 K8 a1 ~# [$ q
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini% B6 ~* L2 q. b( l0 Y4 w3 Y3 @$ N
21 Photooxidation Technology for Correlative Light; E! X2 \: j: D; N4 F' w+ v( ~5 t% A
and Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423& }8 Y% G+ p" \
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,
# \" I/ x1 ]# F: @; I. NMargit Pavelka, and Adolf Ellinger
* O" J2 q% ~# i) H7 u22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 437
/ d2 A+ T1 @" x3 k CCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
) T3 l. g/ I' |) U" y7 j- SMargit Pavelka! L+ _) t7 X! D/ ]% m" {0 W. o
23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 449
0 ]% {. j( K. EKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori. Q( x/ T, H+ {
24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 4670 W3 g1 n+ ?7 a% x0 t4 j
Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
0 Y& A1 B7 S7 h7 I+ d7 } c* f25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 4837 |6 V1 T) e8 x7 e. _+ z% L$ S
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto+ z' p' K4 L$ g+ E1 g' u2 o
26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493
! n6 j. @$ t; x5 uJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
0 p' m8 U, ~# u0 u# [7 |, ?27 Environmental Scanning Electron Microscopy Gold
, P: t; H( w+ E( n+ j3 n4 _1 LImmunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517
4 N5 g' R( L$ Y1 l# V0 OFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
; v* q9 U8 t2 M$ _$ O28 High-Pressure Freezing for Scanning Transmission Electron
/ }" k9 S4 @ JTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 525. {2 `$ ~1 y4 A0 w$ P4 [, k
Paul Walther, Eberhard Schmid, and Katharina Höhn
& b o; D& ` Q$ t4 T! a! X29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 5378 X$ U% p4 a- e4 h, X. F# ] x
Stephan Meding and Axel Walch
9 H8 {2 \4 C3 X9 YIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5475 h( n: o3 w% i% x' h7 O
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