PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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! a9 `5 r2 @, X8 f  E, }: ESpringer MMB Vol931 2013 共29章 552pages. A2 K# V& v4 |2 f: b6 v" H- m
Cell Imaging Techniques  Methods and Protocols( e1 a9 I$ x2 W! d7 o
Second Edition
- ?( ~3 Q0 K0 c9 W9 ?4 K* vEdited by  D, ^" _, r5 \  n$ Q" Q, Z) R+ j
Douglas J. Taatjes
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Contents
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: v2 e3 A9 M3 ^) sPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii4 p: x% |3 o/ Z& X& Y+ `
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii! O! p+ M/ n. J% E- ]% w; |
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
9 D( @1 ~) f/ QDouglas W. Cromey6 C7 y; {5 h6 u/ ^- ?. G2 K( o1 f- d
2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29# s7 E' O0 @3 y
Donna J. Webb and Claire M. Brown
0 ^4 @' h: D" x% e2 J: E3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61* V; `0 I4 r- s4 t9 O6 t
J. Lacoste, K. Young, and Claire M. Brown5 g9 c, K$ P7 P+ ^+ j# X
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
; A+ E2 @+ i# OProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 858 f. X8 R9 @0 ?+ a: |4 b
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
$ I7 o8 o3 n/ U$ T2 Z2 X+ c3 @5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
# C, }- H( y3 w3 H  Y9 ZJeremy Adler and Ingela Parmryd
7 y' u4 @) k/ s# ^% L6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111
) N* ~: q1 y/ ASunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
' Y$ D$ f5 z: L% e7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
" |1 N! m& u1 gRaju Tomer, Khaled Khairy, and Philipp J. Keller# U  F8 x% A% w4 F; @+ r8 X
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1395 |5 J0 |0 b1 N, ~+ d
Lasse Evensen, Wolfgang Link, and James B. Lorens; A% s5 C" A: a* S- S$ }4 |! F" o
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     1533 a- \1 G9 j4 }, ^# A) [
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
( n: G; E* n1 X; W' l10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential # j( D5 t7 ~# N( U# H; h) V
Interference
2 E; _; O% P( |Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169. D; m/ Y/ y2 U+ }$ w% Z( D& d1 S
Wei Sun, Lehui Xiao, and Ning Fang
1 Z1 D7 r$ `: [5 u, C0 \3 V; o. D11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
' d( a" `5 H5 `Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz/ U- A7 t7 ?8 A4 N5 s
12   Laser Capture Microdissection for Protein
3 [' S9 T4 O9 p- r$ ^and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
$ U* ?  v7 T4 E1 H7 R5 GYelena Golubeva, Rosalba Salcedo, Claudius Mueller,
  c) Y& h8 U0 hLance A. Liotta, and Virginia Espina
% T# P3 R, b7 Q) Q" a0 U- D5 I13   Viewing Dynamic Interactions of Proteins and a Model Lipid4 U  F' `% m' ~- T
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259' d0 s8 c# b0 j$ f4 n: l/ d) `* _- ^
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
" k5 F( ~. K4 T+ n1 K14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295( U" T1 r! b3 `) ~
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
' _1 Y4 F: ]- d5 Y* n15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .3130 P% z2 Q3 x* \- b7 B: E% v& P
Joanna L. MacKay and Sanjay Kumar
2 i/ F* b- I1 D2 n16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331: `5 y( H8 e5 W; I$ I# W
Lilia A. Chtcheglova and Peter Hinterdorfer
/ w4 ~% N: Y. t+ Q17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345( \2 u1 g* G$ a/ M7 P8 b
Bhanu P. Jena
9 t! ~6 w$ n! g2 x" k! p( Y18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367$ b3 x# W* v& \* n3 q0 Z* E, S
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
- J' S/ a8 ^) z$ p; |3 P19 A Novel Combined Imaging/Morphometrical Method for the Analysis
- x, a2 N7 ?8 I: ~. g& iof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391, E; m% O0 P0 h( q5 A& u
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
& b! G  y$ b! N; Z+ e0 k20   Correlative Light–Electron Microscopy as a Tool to Study
9 x1 Q1 R: ^: g2 S( UIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413. A5 }' m+ p, ^6 `1 Q
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
" |4 B2 x/ v9 e$ I" I21   Photooxidation Technology for Correlative Light# ^7 A0 U- Q: u, ?
and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423
9 }' t* S, u1 {# X3 Y; SClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, ; o( y( W5 n$ r2 O
Margit Pavelka, and Adolf Ellinger
6 ]) `6 r+ E5 t22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437
9 I- ^3 \6 J! y* @3 zCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
3 \% j/ Q2 j6 g2 l3 EMargit Pavelka
. H" \8 A& ]+ f8 R* l23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     4497 Z. f8 E; E- K+ b
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori4 J. g4 Y  [; h0 M6 l5 B. S
24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
) K; s9 O5 @8 \$ _0 hNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
( r5 W( w1 F! ~0 G8 m4 e8 y; |3 P25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483
+ e  p6 X9 e# ]8 U' Q- L% xMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto) n' J' r6 |- Y% f- S
26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493$ L; Y+ k/ p- ^! q
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
$ s6 W; `& J- C3 ~) U27   Environmental Scanning Electron Microscopy Gold
9 H1 X4 h/ h' a. p) @( Q+ {Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    5170 a9 j+ K7 ^8 i* s5 g* L- u) D
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
, o9 @- D  \: x) |28   High-Pressure Freezing for Scanning Transmission Electron
8 j) }/ n( i% FTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525( d5 c, H4 p- A  I/ [' g) L/ s
Paul Walther, Eberhard Schmid, and Katharina Höhn  \2 n  _  x9 w+ e* c% y
29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537
1 ]8 d, W# b- V# o9 BStephan Meding and Axel Walch
, V* G6 M$ g. Z' H2 W) X5 Y% IIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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