PDF电子书：Ribosome display and related technologies （Springer MMB V805 2012）

xyklmu

$ |* u8 m$ \3 z% p" G. o; F6 j- u6 P2 A/ V5 v
/ `, x& _/ }4 t5 d. q$ X6 \
6 `- j: ?6 d! e! n" R

: s& z6 H. c8 M1 D, f
! F" A, F, I5 V$ Z* _
" k* R# l# B, U& S! c  O5 XRibosome display and related technologies : methods and protocols
( i  G' k2 p8 Uedited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805)   , `7 |9 o. p7 @0 s- g  Z- F/ l
4 [" o" M8 |' A* f
    Contents$ e( t  j% w4 t- f
   
$ D) M& `. D2 b/ z- G" Q" k    Preface
  w+ j( F0 K$ B    Contributors
0 H8 `; B2 e' A/ B8 g, t! o    PART I REVIEW. k; r2 c, U; _
    1 Ribosome Display: A Perspective0 z& S( w3 K7 o2 r# l; g$ D1 \+ x
    PART II TRANSLATION EXTRACT PREPARATION$ u# n/ M( v/ W! d! {
    2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts
9 `  |0 i8 L, o$ `# h$ F' C8 y    PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS, I# G4 q% S5 }
    3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45$ ?, t5 l! X; T) ^. j
    4 Stabilized Ribosome Display for In Vitro Selection, Y+ o0 k( k( V$ c) I& O
    5 Eukaryotic Ribosome Display with In Situ DNA Recovery 750 M# u& m0 K# P* g5 f  c* l
    6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 870 h. t3 M( Z% O3 v* q
    7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101
) T* I: f& ?2 Y  V5 [    8 cDNA Display: Rapid Stabilization of mRNA Display 113
+ `$ B- Q4 [! G( i$ h- f+ {- l    PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS8 T. Z6 `. i1 c8 a! X9 [& V
    9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
; A9 Q. G1 U# a8 c! X/ q' L( b    10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display
2 q. _, k, c) W    11 Evolution of Protein Stability Using Ribosome Display3 F$ D" R. Y3 ], ~! u. f
    12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries6 B% W, _, }$ N+ }$ O
    13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
3 o% Y1 G' b# }$ ]1 K    14 Peptide Screening Using PURE Ribosome Display: ~6 J* i  [4 t7 m: O  S# Z% Q, l
    15 Rapid Selection of High-Affinity Binders Using Ribosome Display 2615 Z; {5 ?0 _  d6 z1 a; L
    16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287) o9 u+ O( f9 Q7 T
    17 Identification of Candidate Vaccine Genes Using Ribosome Display 2999 Z2 E  x3 Z, E+ j% J. U
    18 Ribosome Display for the Selection of Sac7d Scaffolds 315
, k5 g- I. c- F% _+ y    PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS$ E' _# U2 j: C7 o# }' n
    19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335
! h3 y3 Z9 U! ?    20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349% F# _7 N' z1 m, Y0 Y, g
    21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 3675 V! V- G- [0 J# o" c4 d! X3 n% Q
    PART VI CASE STUDIES5 y, d, T* u& q4 z8 F+ C" M( r
    22 Optimization of CAT-354， a Therapeutic Antibody Directed Against Interleukin-13， Using Ribosome Display% m/ E9 v+ r, O- z
    23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 4034 ]+ e2 U0 l# ~1 e
    Index 423
- Z+ o9 P4 A) F/ I  _$ c" t
2 U, r) I% c8 S: G8 Y7 j) |5 j% V[hide][/hide]