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本帖最后由 细胞海洋 于 2013-1-12 22:42 编辑
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7 x3 O g; {" C6 i) |9 URibosome display and related technologies : methods and protocols # @6 K( g/ v1 f5 E0 v5 v
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805) 7 _/ N$ L, t! m$ L }
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Contents7 m/ w" f) ^' e. g
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Preface
+ p0 E3 @0 `% \ Contributors
, t; a1 z2 Q! {7 o PART I REVIEW) d) I$ x1 j! _/ [+ d* D
1 Ribosome Display: A Perspective
# D, q" P" I: S PART II TRANSLATION EXTRACT PREPARATION' m5 D$ n+ V, y8 W/ j
2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts% g* Q. K) Q9 X
PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS r$ N% _$ ~$ [% g& I4 p$ B
3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45
: Y# q- Z/ ?) ]9 r$ B: W 4 Stabilized Ribosome Display for In Vitro Selection+ W: Q0 `9 B" i/ J* b; @/ u
5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75
5 N9 o7 [6 x) k' f! V9 w; ^3 V 6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 874 i( D* d2 S8 ~& d& ], L& ^3 A
7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101
- M) ]7 g& w% q8 Z1 Z 8 cDNA Display: Rapid Stabilization of mRNA Display 113
$ e5 h$ ~! e+ s PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS4 g" r% H, H4 \3 C& U. ]/ P
9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis8 p Z5 O" @& ?9 [. W' G7 h
10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display1 k2 N1 [0 _0 K" w# g
11 Evolution of Protein Stability Using Ribosome Display; S8 Y/ n' n2 w0 F
12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
( I6 N) m/ y( P x! \8 g7 v 13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
; ], l3 {% x; h 14 Peptide Screening Using PURE Ribosome Display5 ~! f) y3 u- {$ |! P
15 Rapid Selection of High-Affinity Binders Using Ribosome Display 261 d% c: `4 r/ ]/ a
16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287
5 A8 O4 x3 E$ ^6 T+ `. b 17 Identification of Candidate Vaccine Genes Using Ribosome Display 2991 _" i' O. c; a2 C% h
18 Ribosome Display for the Selection of Sac7d Scaffolds 315
: ]4 D* D$ E W1 e6 W( ~9 C; {8 V PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS
2 e$ x1 I& q0 Y 19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335
; Z1 {1 H) p9 X, _) r! l) Q5 Y7 p 20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349' w, p( i/ q8 m7 \+ M* L7 ^
21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367
& P1 j: d f# z& p! p: v3 p PART VI CASE STUDIES
" @7 _& k7 Y0 L# t( S* R+ d 22 Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
- E( t' {7 \* K' Q B+ L+ ` 23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 4035 _" s- j4 p$ a4 d0 `
Index 423 w+ h% f5 J/ t
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