PDF电子书：RNA and DNA Editing.Methods and Protocols (Humana Press, 2011)

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& E+ y$ s; l/ V# k* }' l0 N1 B# ^5 DRNA and DNA Editing8 j% L6 [  S* b( W  L0 n
Methods and Protocols% E; [+ ^* Q. Y; S4 J7 v- d
Series: Methods in Molecular Biology, Vol. 718" H/ R; u5 z) W
Aphasizhev, Ruslan (Ed.)
! w6 G- B1 \# d2011, XIII, 275p. 57 illus., 1 illus. in color.
; s' _* f+ D2 k% s7 RA product of Humana Press
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2 ^4 Y9 w1 K' g3 Z0 S5 v' u1 bPart I Uracil Insertion/Deletion RNA Edi ting
( I7 J, T  W  J/ s* Oin Mitochondrion of Trypanosoma brucei
. D& P- G5 ^; b( ]& @) V7 O( E1 Three-Dimensional Reconstruction of Trypanosoma brucei9 k; R# w3 U0 I. f. Q  q% P8 o
Editosomes Using Single-Particle Electron Microscopy . . . . . . .         3
- y$ ~/ ]3 f% ]: e+ wH. Ulrich Göringer, Holger Stark, Cordula Böhm,% M8 J. x+ d# U
Bjoern Sander, and Monika M. Golas
; ^" m7 n; F* t2 iCODA: RNAi-Based Inducible Knock-In System
) m! ^5 s4 f4 h- b  I1 |3 ^in Trypanosoma brucei . .         23
: T6 W! U2 h% aGene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev. I( R% E  K) G1 m5 r
Part II A denosine to Inosine RNA Edi ting  @3 i7 M- N+ O1 X5 t' t
3 Perturbing A-to-I RNA Editing Using Genetics
* D  a5 X- `  U$ e1 m. D' N7 kand Homologous Recombination . .         41/ I! F8 f2 D) ^3 k/ ^' N
Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan
7 T& f5 p' u' S% Q; D# S4 Laser Microdissection and Pressure Catapulting of Single/ Q  _2 t. t' {" p
Human Motor Neurons for RNA Editing Analysis . . . . . .         754 v4 H$ a$ L& A
Hui Sun, Aruna Raja, Mary A. O’Connell, Valerie Mann, Brendon Noble,
- `8 j) a2 {3 a$ |; O9 [1 @and Liam P. Keegan
8 v7 A3 G  p' z( V0 j% _# U5 Biochemical Identification of A-to-I RNA Editing Sites: M; W# v, K5 y
by the Inosine Chemical Erasing (ICE) Method . . . . . . . . . . . . . . . . . . . . .         89
3 D5 o7 _3 b& ^1 m. A4 E  V4 Z/ u7 [Masayuki Sakurai and Tsutomu Suzuki
) ?# F) ]! T5 x8 sPart III C ytidi ne to Uracil RNA and DNA Edi ting1 C$ z2 I4 T3 _( b( u7 F" z
6 Identifying mRNA Editing Deaminase Targets by RNA-Seq . .         1038 P( u: e; n% r+ {0 G4 N- u9 A
Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou
% ^4 s+ ^# [) P: i% I8 X8 g7 Mouse and Other Rodent Models of C to U RNA Editing . . . . . . . . . . . . .         121+ x4 O$ A6 r9 W3 t4 i, T
Valerie Blanc and Nicholas O. Davidson, f% D& k" Q" L7 q8 |( h3 i: i# w
8 In Vivo Analysis of RNA Editing in Plastids . . . . . . . . . . .         137
# s1 V  d8 Z' }Stephanie Ruf and Ralph Bock
  @9 C8 v/ O0 b; K7 W9 Identifying Specific Trans-Factors of RNA Editing in Plant3 A/ h' a; q* U+ P4 d- K
Mitochondria by Multiplex Single Base Extension Typing . . . . . . . . . .         151
0 E  j& x9 [# S) D$ eMizuki Takenaka" B2 t2 p( j/ T, Y, {
10 Complementation of Mutants in Plant Mitochondrial RNA6 R5 I8 `" z; ]: y
Editing by Protoplast Transfection . . . . . . . . . . . . . . . . . . . . . . . . . .         163& I5 K9 U0 `6 G
Mizuki Takenaka and Anja Zehrmann
" S2 w8 W7 W+ U# k' n11 A High-Throughput Assay for DNA Deaminases . . . . . . . . . . . . . . . . . . . . . . .         171% H7 f+ ^8 V: z, k  [$ E
Meng Wang, Cristina Rada, and Michael S. Neuberger
* \' z* x  i3 |7 M% R12 Biochemical Fractionation and Purification5 N% ]" b: `/ b! f& F4 W, u
of High-Molecular-Mass APOBEC3G Complexes . . . . . . . . . . . . . . . . . . . . . . .         185
9 R# |% z$ c/ VYa-Lin Chiu7 G% M5 Z0 d% Z  V# T3 z
Part IV tRNA Edi ting and RNA Modi fications! _2 B0 G0 Q* U5 N, j
13 Analysis of tRNA Editing in Native and Synthetic Substrates . . . . . . .         2093 c' n5 s+ N0 L/ ~4 l
Jessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo
9 V  y) `2 L' v2 r/ [4 S# `14 Post-transcriptional Modification of RNAs by Artificial Box' t4 w+ r- l4 |+ |, S+ j$ J
H/ACA and Box C/D RNPs . . . . . . . . . . .         2273 A1 O5 y; d3 G1 r
Chao Huang, John Karijolich, and Yi-Tao Yu+ |2 j+ `  s% P4 |! D
15 Functional Analysis of Noncoding RNAs in Trypanosomes:
8 ?# e$ H2 r2 O7 h# z" H8 v# ]* qRNA Walk, a Novel Approach to Study RNA–RNA Interactions
( @4 j3 Q6 U( n, E  L% LBetween Small RNA and Its Target . .         245
, R: f& o6 }9 H5 H& `Chaim Wachtel and Shulamit Michaeli
7 O, ?- m- M) M# A16 A Post-Labeling Approach for the Characterization& W7 \0 e- R" e+ d$ I
and Quantification of RNA Modifications Based on
2 }4 H# Y) g7 |) uSite-Directed Cleavage by DNAzymes . . . . . . . . . . . . . . . . . . . . . . . . . . . .         2590 h6 T" ~& f6 ]6 c; D% N/ ^
Madeleine Meusburger, Martin Hengesbach, and Mark Helm
8 K# |$ I8 B8 qIndex . 271
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