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本帖最后由 细胞海洋 于 2013-5-3 11:03 编辑 5 d) z9 |% v7 Q
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RNA and DNA Editing5 b6 b% A( e! {. n
Methods and Protocols1 e- q0 i; o( w$ X
Series: Methods in Molecular Biology, Vol. 718
& R; ]. Z+ D4 r& S* oAphasizhev, Ruslan (Ed.)
6 g/ A4 F4 l- O' H' }2011, XIII, 275p. 57 illus., 1 illus. in color.
/ z2 e& r3 c1 Y/ `% K7 SA product of Humana Press% t' r# F9 x& b
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Part I Uracil Insertion/Deletion RNA Edi ting
0 c4 }8 @6 {9 Xin Mitochondrion of Trypanosoma brucei
& D; v; Q8 [" p. c, h! N& R4 f0 u1 Three-Dimensional Reconstruction of Trypanosoma brucei {6 C- m1 Q* P& x! s
Editosomes Using Single-Particle Electron Microscopy . . . . . . . 3
' B# N$ P. V/ B+ [: mH. Ulrich Göringer, Holger Stark, Cordula Böhm,
* r8 M" |( v3 F9 F7 P8 d* rBjoern Sander, and Monika M. Golas
1 z0 R' ~ Z2 \2 iCODA: RNAi-Based Inducible Knock-In System+ P" h9 v; `7 a! D9 x
in Trypanosoma brucei . . 23
/ g) r* O- A% @9 G; p _, rGene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev
7 C; i7 t, H( u* r) @ F7 QPart II A denosine to Inosine RNA Edi ting
' B0 Z/ H9 V8 d: J5 f! p; a( |( j. k3 Perturbing A-to-I RNA Editing Using Genetics) D, Y( P+ g1 Y
and Homologous Recombination . . 411 ^& \# S' q7 {
Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan# T% f( v8 C+ M3 E
4 Laser Microdissection and Pressure Catapulting of Single- K% ~7 l! p) X4 u$ d1 u% A
Human Motor Neurons for RNA Editing Analysis . . . . . . 75% {- _9 n) V( N" x( g, a
Hui Sun, Aruna Raja, Mary A. O’Connell, Valerie Mann, Brendon Noble,
3 v& k; d5 `. D, K4 {! L7 a- |4 z% mand Liam P. Keegan
) i& Y, Q: C: M- H1 l1 V+ h# Y5 Biochemical Identification of A-to-I RNA Editing Sites
$ q4 h' f7 \3 d: m; M4 f, fby the Inosine Chemical Erasing (ICE) Method . . . . . . . . . . . . . . . . . . . . . 89, |; f; s& R/ s
Masayuki Sakurai and Tsutomu Suzuki
5 ?# w; b6 a/ |, Y9 {4 }Part III C ytidi ne to Uracil RNA and DNA Edi ting( }& z, ^. y% w# d
6 Identifying mRNA Editing Deaminase Targets by RNA-Seq . . 103 r; D! }: a$ q! P
Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou
8 Q& U; A5 A T" P+ X4 s7 Mouse and Other Rodent Models of C to U RNA Editing . . . . . . . . . . . . . 1216 N1 d$ w4 _1 V! W2 s- U# N; N: ]
Valerie Blanc and Nicholas O. Davidson
8 _/ R; U0 K9 m8 In Vivo Analysis of RNA Editing in Plastids . . . . . . . . . . . 137: }. k/ G1 N6 ^$ T
Stephanie Ruf and Ralph Bock
" f: r$ d( X9 Z$ n9 Identifying Specific Trans-Factors of RNA Editing in Plant+ g6 F* a9 j7 w* t7 m4 L
Mitochondria by Multiplex Single Base Extension Typing . . . . . . . . . . 151
. a0 H" k+ x C6 ^1 q$ u; F7 Z# gMizuki Takenaka
$ m! q0 _9 z% E10 Complementation of Mutants in Plant Mitochondrial RNA
4 P8 q9 R* N' [% sEditing by Protoplast Transfection . . . . . . . . . . . . . . . . . . . . . . . . . . 163! _ J6 V: r' B/ O9 n9 {
Mizuki Takenaka and Anja Zehrmann; C5 Q+ g" x9 ?, D9 B) `
11 A High-Throughput Assay for DNA Deaminases . . . . . . . . . . . . . . . . . . . . . . . 171- x5 R9 w- ^7 W/ [/ f/ [
Meng Wang, Cristina Rada, and Michael S. Neuberger
' V$ y7 S8 Z' A+ p7 ~1 V12 Biochemical Fractionation and Purification
& \+ Z$ Y3 r0 j: U2 V7 p lof High-Molecular-Mass APOBEC3G Complexes . . . . . . . . . . . . . . . . . . . . . . . 185
O+ p4 G& P) t- Z5 J! qYa-Lin Chiu+ [& L+ K4 Y& V7 |( [7 @
Part IV tRNA Edi ting and RNA Modi fications
' F Q1 L* h+ W0 V! K13 Analysis of tRNA Editing in Native and Synthetic Substrates . . . . . . . 209
( q+ T/ O6 O, W3 s, sJessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo$ {5 ?4 Q% ? w; o
14 Post-transcriptional Modification of RNAs by Artificial Box
+ G# U5 n$ Y1 ^0 hH/ACA and Box C/D RNPs . . . . . . . . . . . 227) I; e1 q$ A! R$ f
Chao Huang, John Karijolich, and Yi-Tao Yu
' [; X/ R0 Q* n& p. q15 Functional Analysis of Noncoding RNAs in Trypanosomes:
( ~1 W p; o' n7 R; iRNA Walk, a Novel Approach to Study RNA–RNA Interactions8 j/ [8 j* S, l) I. x
Between Small RNA and Its Target . . 245; a3 h7 n: d3 H& V3 ^
Chaim Wachtel and Shulamit Michaeli" w' Z* U; L% v
16 A Post-Labeling Approach for the Characterization
- x4 N% H5 B/ _1 f1 Land Quantification of RNA Modifications Based on# T$ k* @" l2 Y- b
Site-Directed Cleavage by DNAzymes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
" Q+ V, K' V3 M" b7 TMadeleine Meusburger, Martin Hengesbach, and Mark Helm6 Z5 M$ b% B7 y* H' e7 \" h& s' U
Index . 271" y# J; c6 k$ D: {* j/ {
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