PDF电子书：RNA and DNA Editing.Methods and Protocols (Humana Press, 2011)

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RNA and DNA Editing. r: l& R! a( F' h
Methods and Protocols+ l, g( G: w( v0 ?- Z7 A0 F" k7 u7 N
Series: Methods in Molecular Biology, Vol. 718& {: i: A8 ?6 g  t2 ?7 Y; y6 ~
Aphasizhev, Ruslan (Ed.)
. V" `* M  Y- X& W6 w/ \9 }2011, XIII, 275p. 57 illus., 1 illus. in color.3 I4 M7 P4 q; Y. E1 A: M0 ^
A product of Humana Press
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& V3 j/ \, b7 J( \Part I Uracil Insertion/Deletion RNA Edi ting
" T. _. Z4 t9 A+ s6 q! fin Mitochondrion of Trypanosoma brucei/ Z  j. T: N+ k/ f$ Y- G# }; F
1 Three-Dimensional Reconstruction of Trypanosoma brucei
6 v: U6 b7 W1 Q4 b) c* `Editosomes Using Single-Particle Electron Microscopy . . . . . . .         3
) k6 e0 J, T3 l0 L* S; v: T" nH. Ulrich Göringer, Holger Stark, Cordula Böhm,
$ z# a6 o* s- ^  g: ~Bjoern Sander, and Monika M. Golas/ |+ H' |) F+ L7 W, K& \4 O
2 iCODA: RNAi-Based Inducible Knock-In System
: Q$ O2 v7 w! v/ tin Trypanosoma brucei . .         23
! @5 h  H  D' ^4 q2 NGene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev
' }2 c7 q& j' Y( \! }/ lPart II A denosine to Inosine RNA Edi ting5 d7 h, {9 e5 m. L4 ]- i9 e. C% r
3 Perturbing A-to-I RNA Editing Using Genetics* ^5 R. n9 {' [1 E
and Homologous Recombination . .         41/ v# L! b9 i, l% P0 U2 Q4 B( P$ j
Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan
' ?) c. A9 ~! D7 B' o# }2 @1 {4 Laser Microdissection and Pressure Catapulting of Single
$ ^  G  {' X* U5 ?Human Motor Neurons for RNA Editing Analysis . . . . . .         75' i3 g. z$ _, t+ q1 n5 v: z
Hui Sun, Aruna Raja, Mary A. O’Connell, Valerie Mann, Brendon Noble,
8 N: D/ j# n' B+ m7 r( V! Vand Liam P. Keegan
7 B. k1 c+ f* Z/ }5 Biochemical Identification of A-to-I RNA Editing Sites8 L  R3 B, E* k6 R; t
by the Inosine Chemical Erasing (ICE) Method . . . . . . . . . . . . . . . . . . . . .         89  E/ j: b! a; h8 F0 n3 r' x
Masayuki Sakurai and Tsutomu Suzuki: Y- B% |, R( W0 v
Part III C ytidi ne to Uracil RNA and DNA Edi ting
6 b" T- G- l* k. k; |. S6 Identifying mRNA Editing Deaminase Targets by RNA-Seq . .         103: I0 x" f1 ?4 Q! J- [/ e% ]% K5 ^
Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou$ a9 e: a2 R  W9 ?9 k3 }9 q
7 Mouse and Other Rodent Models of C to U RNA Editing . . . . . . . . . . . . .         121/ G: H" P* k, Y' Y" v' h
Valerie Blanc and Nicholas O. Davidson3 Y7 X; Z" Z" U0 ^
8 In Vivo Analysis of RNA Editing in Plastids . . . . . . . . . . .         1372 N5 f" F( i3 ]; y( U6 \* x
Stephanie Ruf and Ralph Bock+ D$ @! A1 |7 T7 k5 B
9 Identifying Specific Trans-Factors of RNA Editing in Plant; @6 X% q  e5 P
Mitochondria by Multiplex Single Base Extension Typing . . . . . . . . . .         1516 P7 w9 I" `' F
Mizuki Takenaka
4 n' o) A% x/ Y& z: o10 Complementation of Mutants in Plant Mitochondrial RNA7 p2 O, \/ k0 f# f' @. e
Editing by Protoplast Transfection . . . . . . . . . . . . . . . . . . . . . . . . . .         163
" j& ]; x( W3 g, tMizuki Takenaka and Anja Zehrmann' z" y! M  E, D, U7 p5 I( k
11 A High-Throughput Assay for DNA Deaminases . . . . . . . . . . . . . . . . . . . . . . .         171
+ s/ t& [& w9 \2 UMeng Wang, Cristina Rada, and Michael S. Neuberger
2 e" `" z3 |4 q  {' |12 Biochemical Fractionation and Purification. L1 H" M* }+ ^7 e
of High-Molecular-Mass APOBEC3G Complexes . . . . . . . . . . . . . . . . . . . . . . .         185. r& ^/ s1 T* }% e) ^( ~- |
Ya-Lin Chiu8 n3 L2 K# R& b0 f7 s
Part IV tRNA Edi ting and RNA Modi fications
' G2 v/ D( l# e2 k13 Analysis of tRNA Editing in Native and Synthetic Substrates . . . . . . .         209
# Z- O# M0 P% w9 pJessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo; I" p& l; r! Y* p6 T$ K
14 Post-transcriptional Modification of RNAs by Artificial Box
! j4 W, p+ g8 P: ]9 HH/ACA and Box C/D RNPs . . . . . . . . . . .         2275 ^' {+ X% B, r4 u1 i* F
Chao Huang, John Karijolich, and Yi-Tao Yu
; u: f; V) Y) R15 Functional Analysis of Noncoding RNAs in Trypanosomes:
& C) q, @$ w. d& nRNA Walk, a Novel Approach to Study RNA–RNA Interactions
5 D2 c( Y+ ?) r3 H/ Z( ^# G; mBetween Small RNA and Its Target . .         2451 V# Q2 V* H! ~8 s5 D
Chaim Wachtel and Shulamit Michaeli
  |+ P5 x  w7 m! X0 j; P" c! @16 A Post-Labeling Approach for the Characterization
6 B& I& H4 [. Fand Quantification of RNA Modifications Based on1 S1 S+ S) H/ O1 z
Site-Directed Cleavage by DNAzymes . . . . . . . . . . . . . . . . . . . . . . . . . . . .         259; p5 z2 h0 Q: m6 J' l( Z# C
Madeleine Meusburger, Martin Hengesbach, and Mark Helm8 s) Z, K7 n8 b2 B: C( W/ `
Index . 271
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RNA and DNA Editing.Methods and Protocols

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