PDF电子书：Recombinant and In Vitro RNA Synthesis

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Recombinant and In Vitro RNA Synthesis6 |! h; d4 L: y; y% |
Contents" j7 y- |0 R5 ~' t0 {8 c
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v  w, E6 G; p4 r6 C5 ], r
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
: P9 Y7 p4 \* m/ Y' {1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1* H0 Q8 W( }0 `# ]" A
Luc Ponchon and Frédéric Dardel. r$ h! c# {  y5 r' w$ j8 X
2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 9" t( w( |% `' O1 `: Q2 C" g
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,0 R5 {* V7 K5 Y7 g8 a8 T( A
and Frédéric Dardel6 n0 g, X, y. E! ?1 t: r* M
3 Preparation of Long Templates for RNA In Vitro Transcription
7 o7 n* @* {2 R% jby Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19$ I# S7 d* Q& R: c* i; R, @2 w7 m8 e
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,! F7 ]! t# l% K% k- m/ l; E1 o
and Loren Dean Williams
% H* ?6 @# N5 H5 T: e/ b4 General Protocols for Preparation of Plasmid DNA Template,( U* X/ `" {& U) U1 x1 r; \" V
RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 43
7 r* C) R6 y' e1 {Jo L. Linpinsel and Graeme L. Conn& ]  J7 w$ Y$ z4 q
5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59
, y! ]' e  U: r" L; gCheng Lu and Pingwei Li
; A* j' B" Y6 a$ D5 Y; W& N1 @6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 69
1 ~  [1 _1 |5 u; a6 u$ r$ E7 }+ FEvan P. Booy, Hui Meng, and Sean A. McKenna
" k) s9 W" G! h7 Cis-Acting Ribozymes for the Production of RNA In Vitro
! {5 R" q9 X) X+ b6 c' d8 n9 P' DTranscripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83; h5 L, q5 J5 D3 z) I0 M
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker" _4 H4 B( A* L2 T# u, _# `
8 Trans-Acting Antigenomic HDV Ribozyme for Production7 \. R7 Y3 j* Z+ A6 t8 j& Y) n
of In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 99
, d8 X0 T" ~! v$ LMilena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,
/ h& y2 Z7 y. z) \# ~+ @5 Z9 r. wand Jerzy Ciesiolka2 W2 r& o: e* ?; r6 s8 X
9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography/ V+ ~8 z- n) d+ f
and DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
3 @- N( n6 d3 t7 [+ VHae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong) q% ?& ~; T% V% ~8 W4 d% Y
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 1239 p+ n* D. \# n& W" }
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,' l" I$ y  R0 f2 T2 D' e
and Pascale Legault/ O. ^/ b) T. ]" e* j
11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137- I" C1 i6 e% c- z8 R  Y% y: ~
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,' ?5 @; z/ H% ?" y; i; N
Alexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault
/ U. ]; x9 L; p5 f7 x; h2 ^3 C12 Plasmid Template Design and In Vitro Transcription of Short RNAs
( V5 t/ ?# B4 ?& @- ?3 |Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157
5 Q9 e* H1 u* z: t2 N13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171  n9 i8 Q) y4 O. d4 |& g* j4 o
Stephanie L. Moon and Jeffrey Wilusz
- b' ^- |0 v5 Z- V; ^14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181
1 E# k% y# B! Y+ \; B9 h1 WN. Ruth Zearfoss and Sean P. Ryder
% t) Q5 ]1 ^7 u4 q1 a15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 195, d  `/ e0 \" e  u: R6 a' E2 B
Ya-Ming Hou
7 F+ v7 Z. u* ^) B16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213
! S/ f9 |/ B5 }0 d( CLina Lin and Zhen Huang
. l5 q; N% b. o  F. S" C+ t- O) b17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution+ P9 s7 T: @0 I# J8 ^
NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227' `2 B) K5 R7 v: H% V6 I8 y5 Y
Luigi Martino and Maria R. Conte, |) _* h/ Y0 ]& E( d+ h
18 Preparative Separation of Ribonucleoside Monophosphates
- q1 x2 E% A7 M1 X! Z. nby Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2474 n; B% y3 ]1 v$ ?& o. d
Pierre Dagenais and Pascale Legault
/ n$ d6 t  @: `! q( u" [19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257
, P% t3 Q! c; ~5 K6 E& eChristopher J. Kershaw and Raymond T. O’Keefe- V5 B" X1 T9 @8 |
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
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