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本帖最后由 细胞海洋 于 2013-5-7 09:33 编辑
9 w- ~( o& o$ M: c- @
& L7 ^: x! U" G- ~& s, E7 FRecombinant and In Vitro RNA Synthesis
8 D( a' [ ]- }- e! B; LContents8 T% W# k% f: Q$ b# T
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
/ z, K Q/ S" w3 h: CContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii: l( h0 D6 e+ k f7 \
1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1
[& m. @9 y d9 g, OLuc Ponchon and Frédéric Dardel
- c! H. D9 R2 i* f2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 91 f& c0 ^4 |% }- b& V
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,
) E! q& e$ o& k# w+ Zand Frédéric Dardel/ }9 _- h2 s4 [, c( j
3 Preparation of Long Templates for RNA In Vitro Transcription# _8 s+ M ]9 R+ U2 c
by Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
" C+ S! v! R: R9 b+ MJessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,
( P% u! b. [# W6 _) k) [) band Loren Dean Williams/ I3 |0 ?- x7 [% e& f) \0 I
4 General Protocols for Preparation of Plasmid DNA Template,
/ W9 u) u" J6 j8 H; I! D8 @/ pRNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 43! T, C( |7 ]! @; D, Y
Jo L. Linpinsel and Graeme L. Conn9 n# c4 N" R* Q; o8 O
5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59
4 f {" [& R- `) Q% [6 w4 mCheng Lu and Pingwei Li( f. n- W. c e7 H6 `0 f
6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 69. U+ E3 Z0 O, S
Evan P. Booy, Hui Meng, and Sean A. McKenna4 j5 ?' u7 z( f8 Y% }) u
7 Cis-Acting Ribozymes for the Production of RNA In Vitro
* [5 s& Y! Y3 M+ F7 qTranscripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
0 }/ h' R' M9 u# O3 t9 ~Johanna M. Avis, Graeme L. Conn, and Scott C. Walker. h ?" y( }3 U1 A# s
8 Trans-Acting Antigenomic HDV Ribozyme for Production& ? [9 w+ g' h7 s+ o
of In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 99
$ `& @; y5 v8 B+ o* D: |Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,
: a- M, [. A% C- T, r1 eand Jerzy Ciesiolka
5 d6 J1 E2 r: |, v' Q# T! w& Q9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography
% k O- I3 n8 J$ B, o. Dand DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1134 r7 E! N! M5 m( u% T0 n
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong2 ~( X$ f3 ^0 n; a
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 1232 P6 G$ c U, A4 I& s( E9 i {
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,2 C; \, T; f( I: m! @
and Pascale Legault+ Q6 r8 v1 a, C; U' u: z
11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137
8 x7 a }( @; Z$ l. gGeneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,; I* R( x, W: Z7 D! U
Alexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault
, V0 t( p7 x0 z8 B12 Plasmid Template Design and In Vitro Transcription of Short RNAs
' {4 c7 [, c6 k. L" aWithin a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157
$ h9 ~- I, N _: {7 R; m% W+ h13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
" v% ?9 |# C, q5 C/ H0 uStephanie L. Moon and Jeffrey Wilusz2 i) w" I$ L+ Q* V; ^. s/ r
14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 1810 F0 F1 R2 a6 Q2 |" X* b6 ?
N. Ruth Zearfoss and Sean P. Ryder0 J) z7 Z6 ^& `- @) ]4 D
15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 195
4 x$ V! w0 E$ Z& J( V5 QYa-Ming Hou
n j, J) n) W1 e16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 2134 g: m1 ~5 T) Q9 X
Lina Lin and Zhen Huang
* }' x; C2 _5 ~7 Y! h. D17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution
3 S2 v+ L5 X" ~4 ^- V) ]NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
; s! [6 ^* K( z& ^# _4 z7 rLuigi Martino and Maria R. Conte
1 Z4 `0 \0 c% m3 Y6 B8 c+ m5 L18 Preparative Separation of Ribonucleoside Monophosphates
6 A8 G& Z3 y+ s' a! ~by Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247; N4 E! ^! c, y3 ?" C. {
Pierre Dagenais and Pascale Legault5 O+ k I% x/ `
19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257 W# A4 a5 p) t( F! Y" \
Christopher J. Kershaw and Raymond T. O’Keefe* n2 u- J* E ?- ~: o. c8 n% f
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271! Q! l3 r& W* ?6 q2 Q
6 ~6 z2 a7 R5 Z* _
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发表于 2013-5-6 12:53
