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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑
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; m, D2 W, n3 C8 e- N5 j% |' ]《神经生物学实验操作手册》目录, Y, g( W( r, w5 i+ ]
简介 ' Q) q# d! `/ W1 m2 N# Y
神经干细胞及神经发育 ······································································5* Q. S' C. I3 }" V
神经系统疾病中的神经细胞类型 ···························································8
0 [" P( |1 @9 d1 I( `神经细胞培养及分化
& j, x# M2 n: j5 b5 r$ n+ Y 人神经干细胞培养 ············································································ 11
& V; W7 Q" i% s z" j 大鼠胚胎神经干细胞培养 ····································································15
9 ~ ^) p: |7 s7 Y1 M- h 神经干细胞的无外源成分培养 ·······························································19! K( R# T- K7 t/ `
人多能干细胞诱导成神经干细胞 ····························································21! j3 V8 t- h2 i
神经干细胞分化为神经元和神经胶质细胞 ················································31
! B% w4 ?& O @' c3 n. [1 A p! G- X; Y 神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
% }! {! P; ? P5 T7 I; |8 W2 T 皮层星形胶质细胞的诱导和培养 ···························································41
$ U# o2 G% w, o! _- u" o/ o+ m 皮层及海马神经元的分离、培养和鉴定 ··················································43
) H; {) t7 @+ ^9 Z4 K: Z* ~& i 多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································48) V+ S+ ]2 q! u X" t/ S1 U
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56
. q5 M$ J2 D3 ^$ s' w( v" [ 神经干细胞的冻存 ············································································60' Z3 i) Q! s) h8 j
冻存及复苏成熟分化的神经细胞 ···························································62
N# C: O3 D) S! c( E% H7 z% `: ` v细胞分析 3 U- B! g/ V0 N J& K% d$ \
神经干细胞的细胞活性分析 ·································································64
+ b+ d7 G* M. z/ H5 s# Y- A, z 神经亚型特性标志 ············································································66
/ A) S& ~; N1 L6 ~( A2 L) x7 A, [ 表面标志流式细胞分析 ······································································67) Q7 R7 T1 a/ n2 ?
免疫细胞化学分析 ···············································································69
- M9 e& \' s1 ?( S/ W使用Fluo-4 进行细胞内钙流检测 ······························································729 N" c- T+ Y( y+ T# ^ F
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
