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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑
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《神经生物学实验操作手册》目录
/ e2 t U- w" n0 W简介
, j! d: [% L4 D& h% p- d, n- G$ o 神经干细胞及神经发育 ······································································57 s4 U, h# C! c4 c) b
神经系统疾病中的神经细胞类型 ···························································8$ p% _( }% p$ {+ }$ ?
神经细胞培养及分化 7 h# h, l! E: _8 W3 B/ i* J3 v
人神经干细胞培养 ············································································ 11
8 b% ]" E1 n. l& N4 H( B 大鼠胚胎神经干细胞培养 ····································································155 b7 L6 h. _, d- x+ \, g8 |
神经干细胞的无外源成分培养 ·······························································19
1 S- U2 o* c. P) u5 P 人多能干细胞诱导成神经干细胞 ····························································211 U0 n7 s# `5 C
神经干细胞分化为神经元和神经胶质细胞 ················································31
6 v" z- a+ P' | 神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
4 U& @4 k5 d6 o: d9 r 皮层星形胶质细胞的诱导和培养 ···························································41; P6 p( {3 L/ w$ \2 E9 B0 D
皮层及海马神经元的分离、培养和鉴定 ··················································43: u! K) s2 r* E- m! z6 {# t; M9 l/ D& e
多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································48 l7 f7 z# v. H7 m
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56
7 X0 W0 E0 O4 \8 H: P; [ 神经干细胞的冻存 ············································································60
* z6 c- d# p$ y 冻存及复苏成熟分化的神经细胞 ···························································62. F9 }) _4 n# N: A ?
细胞分析 $ J$ a# J' w* ?' }8 T/ K! o. }
神经干细胞的细胞活性分析 ·································································649 w$ {8 ~- H3 Z+ r7 V
神经亚型特性标志 ············································································66
4 n/ a9 D& _: ?# g 表面标志流式细胞分析 ······································································67" Y7 h' u4 q" |7 x
免疫细胞化学分析 ···············································································69
2 x, b' H1 e* W: S ~. D& c使用Fluo-4 进行细胞内钙流检测 ······························································72 }; O9 ?* K3 Y- |
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
