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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑 : _, Q- Z5 G; f& B$ l
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& U$ T" u k* n5 m《神经生物学实验操作手册》目录' F; u: M/ {+ M& |$ \9 U
简介 & @& ~# s7 t6 R) J r7 f8 ?
神经干细胞及神经发育 ······································································5
) `9 T! e1 l# H 神经系统疾病中的神经细胞类型 ···························································8# f( v/ J7 G3 k7 d" B1 P% ~4 X
神经细胞培养及分化
3 Z& @8 y$ R4 D( O9 Y 人神经干细胞培养 ············································································ 11
/ x, [8 l# x; q+ O3 u 大鼠胚胎神经干细胞培养 ····································································15
9 K1 |9 |! `" J- P* u2 h# I 神经干细胞的无外源成分培养 ·······························································19, ?4 n$ \' R# C. g2 O9 ] U8 l: P* J2 V
人多能干细胞诱导成神经干细胞 ····························································21
: ]" f2 K9 B1 ]1 X0 b; Q3 N 神经干细胞分化为神经元和神经胶质细胞 ················································31# G2 z5 a0 F/ v" R6 [9 E8 J6 @
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
% J! V' x) i# L; T- d% i! H 皮层星形胶质细胞的诱导和培养 ···························································41
9 ?" W3 w9 U2 o) i2 |$ k, I 皮层及海马神经元的分离、培养和鉴定 ··················································43
% ~2 x' W8 i0 T, {4 [; B% w 多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································484 b! p+ A# c- m( j5 H" N J/ ]4 B
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56' ~. t4 c$ ^5 v" N# m( b3 v
神经干细胞的冻存 ············································································60
3 |8 u0 X5 o% ^. X2 n ^ 冻存及复苏成熟分化的神经细胞 ···························································62
5 p& D- i! o" J0 T2 m2 c细胞分析 ! m' k& w2 w3 |5 q+ W8 ~
神经干细胞的细胞活性分析 ·································································64
, U i6 }' z8 h" A; \0 H 神经亚型特性标志 ············································································66) Z2 u4 I1 H0 R( Q2 W
表面标志流式细胞分析 ······································································67
7 ~6 \- u6 O0 H' |; _ R免疫细胞化学分析 ···············································································69
* V/ s ]& w) o. M) t使用Fluo-4 进行细胞内钙流检测 ······························································72
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
