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本帖最后由 细胞海洋 于 2014-1-4 09:43 编辑
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Molecular therapies9 }0 x% y& _; v4 C8 m' `
Dr. Zoltán Balajthy, Dr. János Aradi, Dr. Zoltán Balajthy, Dr. Éva Csősz, Dr. Beáta Scholtz, Dr. István Szatmári, Dr. József Tőzsér, Dr. Tamás Varga (2011)/ g: o, t: q! u+ k$ `) y9 u
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University of Debrecen- p' d7 [& U& O! ~ J- u
Copyright © 2011 The project is funded by the European Union and co-financed by the European Social Fund., Manuscript completed: 17 November 2011, ^. s+ f$ j% J% O8 R
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/ C! L1 A: U, W* ^9 t: DIndex, i8 {( f5 t( M$ I
1. 1. Functional Genomics" b9 L ]7 X, R/ i" m; [
1.1 Definitions
& S* [0 q9 A. g* {$ E1.2 About diseases- @, M6 g: k3 [+ D2 h4 ?3 L" W' x8 ?
1.3 Approaches to understanding disease mechanisms& _ m# X" ]/ Q1 x9 P+ M0 ?
1.3.1 Gene expression is regulated in several basic ways' m4 S2 x1 \* u: L
1.3.2 Microarrays: functional genomics in cancer research
- z2 f1 t; d5 p1 p1.3.3 A Variety of Genetic Alterations Underlie Developmental Abnormalities and Disease
) v1 I2 J/ Y9 Z1.3.4 Genomic microarrays
* I( T" J. t; ]( n7 X2. 2. Recombinant proteins) Y" M* [ y+ {' V! Z" H
2.1 Overview: Protein pharmaceuticals6 ] r2 q/ |" ]1 t
2.2 Cell-free systems: In vitro transcription and translation8 g3 S+ M0 e$ O3 C
2.3 Recombinant protein expression in isolated cells (cell culture)
' w, M9 F; g3 K. L8 Y+ R6 K" Y0 m2.4 Non-prokaryotic expression systems1 z- r8 H8 N [* E7 F
2.4.1 Cloning in Pichia pastoris+ [. q. J+ q- I" t2 t# a
2.4.2 Baculovirus mediated protein expression in insect cells
7 S( o5 b/ q. c& z$ w2.4.3 Mammalian expression systems/ {' X6 e q' S+ d5 ^3 R
2.5 Purification of recombinant proteins
' A2 t3 V2 o! f6 K; t8 _! c3. 3. Gene Therapy: Vectors and Strategies.- I' h, h% g2 w& z# S3 Z$ |
3.1 Vectors for Gene Therapy' G0 r( K$ V) G# h( B4 S) K5 D
3.2. Types of gene transfer, vectors for gene therapy- J) H( b, J* q) J9 P+ c
3.3. General gene therapy strategies* l% T, r7 Y) v) S* V
3.4. Human gene therapy
4 @1 A2 ~( H- z6 P& X- W2 m$ W4. 4. Protein replacement therapies.& ~+ J' L( `+ W. l' u' R' c
5. 5. Recombinant antibodies and the phage display technology' c; m; Y ]) h
5.1. Introduction
, [; Q0 R" x1 Z; J4 W5 A3 W0 A& b5.1.1. The structure of antibodies and their production in the body, l6 M, v+ x" ~ U( U
5.1.2. Antigen-antibody binding( D/ u0 u+ @: r
5.2. The production of therapeutic antibodies
. Y/ O3 N$ J( V5.2.1. The production of antibodies in hybridoma cells.
. @% U+ G- J. }7 l; K; h5 g+ M5.2.2. Humanized antibodies
4 Q' i8 |* W' v; [+ w% w: T5.2.3. Production of human antibodies: ^8 `* L; J. E7 P
5.3. Generation of antibodies by phage display8 ]' z8 [) p6 B$ r( q
5.3.1. The phage display technology' d. W9 H) P7 w' p
5.3.2. Generation of phage libraries/ X2 x8 s- y1 k0 |
5.4. Administration of therapeutic antibodies1 ~; i4 Y, w$ d) \/ f1 R" p y7 q; Z
6. 6. Anti-cytokine Therapy (sepsis).
" q' w E2 z- l4 C$ i; ~5 Y0 }7 |6.1. The consequences of developing inflammation; s3 U+ l/ {' B; h
6.2. Development of Inflammatory Response: Synthesis of Lipid Mediators
4 x+ U# c( }! N/ E" a2 }+ n( W1 O; q6.3. Role of the Liver in Maintenance of Homeostasis: Acute Phase Response
3 _0 V9 j5 ]/ H) n6.4. Time-Course of the Inflammatory Response During Sepsis
0 J9 H9 [" ^0 g0 q' w, K9 M& A) a7. 7. Animal models and transgenesis in biotechnology
) d- w% l8 Q3 o7 |: ]5 k' q- p8. 8. Embryonic and adult stem cells in regenerative medicine I.$ h- e- h; }/ \9 E$ J
8.1 Embryonic stem cells0 U @1 z! r, L2 D
8.2 Somatic cell reprogramming into pluripotent stem cells3 B6 A# M% D! Q d/ `# g+ w; F' q
8.3 Adult stem cells1 T+ S# O& E" ]' v; H$ G& Y
9. 9. Embryonic and adult stem cells in regenerative medicine II.! o6 O) v3 \0 V% z) l& V: M+ X/ z
9.1 Pluripotent stem cells for regenerative medicine
7 p% v- f* Q. u/ n) C7 O# i9.2 Clinical application of stem cells
4 f) O. G" u/ e( Y9 B, K0 Y9.3 Stem cell therapy to cure various diseases
) c; o% {1 X. w$ B/ e7 B10. 10. Cell Cycle and Cancer Therapy, p53 I.
, r" ^/ w1 T( S3 b10.1 Cell Cycle
- a: V7 i2 p G6 y% [ b10.2. Mitogenic Signaling in Eukaryotic Cell Controls the Rate of Cell Division
) Y9 p- e" o! \, C10.3. Biochemical Events of Cell-cycle – in M Phase& y: w! L( v# y, ~, K( {
10.4. Protooncogenes9 s. g8 A) P' V; S' ?1 ~
10.5. ErbB/HER Receptors4 }5 M2 x7 ?6 Z0 m7 ]
10.6. Therapeutic targets5 q; j& ~% J$ c& L& N2 T: d* v
11. 11. Cell Cycle and Cancer Therapy, p53 II.1 C9 Q: P# c5 g- U* y! ?
11.1. Tumor Suppressor Genes and p53# G2 }: e4 E1 A& \! Y* V! P
11.2. Biochemical Pathways of Apoptosis and its Therapeutic Utilization. j6 O+ Q2 H8 K$ c' B+ i- E
12. 12. Gene Silencing Technologies.
! V% [% i& ^( V$ F+ n4 Q12.1 Introduction. e. S9 F! h- S& e |
12.2 Action of antisense oligonucleotides% y8 T! E8 r7 N5 k
12.3 Chemical modifications of gene silencing oligonucleotides; general considerations
( l+ z9 [5 `" d: u) Q12.4 Inhibition of transcription by triple helix forming oligonucleotides
4 n% v! r3 B6 h" r12.5 Gene silencing by ribozymes
/ i" C2 r% ]* _: B' q/ |6 r, w% L12.6 Gene silencing with short RNA fragments% T [! w& n9 i
12.7 Important final note# n/ D C5 i! S4 K6 |' {# l
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发表于 2014-1-3 16:18
