请高手指点这段话是什么意思？谢谢

lingli318

最近看文献，文献中这段话我看了几遍不知道什么意思，虽然自己之前是学生物的，对这方面接触的还比较少，想请高手帮我看下下面这段是讲什么的？什么意思，万分感谢！( {, v& @9 }! v6 b
The CMV promoter element was removed from the FLAG tagged tetrameric wild-type EosFP  by restriction digest with BglII/KpnI  and the annealed and phosphorylated oligos of the miniCMV promoter sequence (bp
51 to +77 of the CMV promoter) were inserted. This construct was then digested with ZraI to allow insertion of the annealed, phosphorylated and ligated HRE element oligonucleotides. Confirmation of HRE copy number was confirmed by digestion with KpnI/SspI/XbaI andthrough DNA sequencing by the Australian Genome Research Facility .

lingli318

回复 whs1230 的帖子' Q2 T: t; U* Q3 \+ F" \" k

! v4 _, r. k8 t1 e7 e' R  s' v谢谢，我大概知道什么意思了，请问这个实验过程是不是非常麻烦呢？

lingli318

回复 tianzhang 的帖子
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谢谢指点