求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

未来之星

3 H1 {4 I* Z8 N  E
) v0 B& ~8 F" L. H
Carboxyfl uorescein succinimidyl ester (CFSE;6 x$ t! o. z- n1 U/ Z2 ]
Invitrogen)-labeled PBMC were cultured at3 P* D$ H4 y2 ], \: F
400 000 cells/well in a 24-well plate with cultured,
+ P8 {4 m5 _1 F( cfreshly thawed or paraformaldehyde (PFA)-fi xed
$ z& ~& ~- E6 LMSC at a ratio of MSC:PBMC of 1:3 and 1:9. T
  y6 V$ z1 B' E' K8 R% xlymphocytes were stimulated using 0.4 μ g/mL antihuman. j/ l- L3 p8 U. E8 B8 R% p* M
CD3 and CD28 antibodies (clone HIT3a! G1 j4 f, [$ L! ?/ Y( Q3 U
and CD28.2, respectively, from BD Pharmagen,
! ~; F. a8 I# `" r6 k2 DMississauga, ON, Canada). T-cell proliferation was  K& Q0 f" k# v* V4 c& E, q
determined 4 days later by fl ow cytometry analysis; j( E& L! Y, W9 d- Q& [( L
of CFSE fl uorescence intensity.
1 R% a) `  }& Y9 C3 _  d
% F. K( ^; a! Q; Gparaformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

cleargreat

丝裂霉素是处理PBMC的吧。

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回复 cleargreat 的帖子/ X0 k' _6 m# x( j

5 a2 {) X  e' R9 M  w如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

cloudycity

这说的是PFA固定过的MSC吧···
( a% P! r. C/ K; Y7 `/ Y0 E/ f但是一般灭活都是用丝裂霉素或是射线灭活，PFA还真没用过···

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! i- u" G9 M* n+ vMSC为何要用PFA固定呢？