|
 
- 积分
- 421
- 威望
- 421
- 包包
- 1962
|
本帖最后由 细胞海洋 于 2015-3-18 10:03 编辑
- D* F) a9 A6 s: v( y' D2 u- y& U) T0 ~$ f; Y8 }
Carboxyfl uorescein succinimidyl ester (CFSE;
. O5 w+ X' a8 f# L0 QInvitrogen)-labeled PBMC were cultured at
3 r5 Y" ^, G2 E$ y400 000 cells/well in a 24-well plate with cultured,
) S$ O( n" A' q2 t7 {freshly thawed or paraformaldehyde (PFA)-fi xed a6 a" {: { x4 A% C. [) r& T
MSC at a ratio of MSC:PBMC of 1:3 and 1:9. T
. a, \& e% A5 T+ J4 Q, \6 A1 ilymphocytes were stimulated using 0.4 μ g/mL antihuman
5 H" R1 d9 Q/ ^# \, ZCD3 and CD28 antibodies (clone HIT3a4 e: ?5 b4 L$ V5 K9 R7 }& U
and CD28.2, respectively, from BD Pharmagen,
0 ^9 [1 U& S' W% [0 S& y, I) }Mississauga, ON, Canada). T-cell proliferation was
( N' ~' N1 }8 ^8 zdetermined 4 days later by fl ow cytometry analysis! b. c, {5 G6 J( L. F3 A
of CFSE fl uorescence intensity.
: A# f* b* H4 C7 h& a0 W2 }% N& I" t j& I9 @
paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？ |
-
总评分: 威望 + 1
包包 + 5
查看全部评分
|
发表于 2015-3-18 08:52
