PDF电子书：Cancer Cell Culture Methods and Protocols（SecondEdition）

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Contents# N. ]& k; ^1 ?
Preface.............................................................  v0 p  y+ F9 e4 o$ @( n
Contributors.........................................................  xi
6 `; M, ]6 O. ]3 p* @7 I  1  Cancer Biology ...................................................  1
" [0 h$ n" B* E* k; QIan A. Cree
1 R6 {1 O( D  i  2  Principles of Cancer Cell Culture .....................................  13
7 j6 [. M) w( lIan A. Cree
! w& U- w! D& n$ V3 [9 [8 }  3  Storage of Cell Lines...............................................  27
( X& t* A) s+ I: HKatharine A. Parker , C% [# J$ e: I; l! ~
  4  Characterization and Authentication of Cancer Cell Lines: An Overview ........  35
' ]; ]1 d0 T+ |* j7 I2 `Yvonne A. Reid * C% R) |( h+ X9 e9 N
  5  Online Verification of Human Cell Line Identity by STR DNA Typing .........  45
9 z2 T3 v0 g( P1 SWilhelm G. Dirks and Hans G. Drexler
& X9 n1 E2 t, b+ Y' N9 G0 q3 M  6  Cytogenetic Analysis of Cancer Cell Lines...............................  571 j+ V4 D. c' @8 a$ ^
Roderick A.F. MacLeod, Maren Kaufmann, and Hans G. Drexler
; G2 y+ I. ?+ ~  7  Cell Culture Contamination .........................................  79; |, D7 k. B  Y& O' e" p
Glyn N. Stacey
  z$ g$ t8 @- y) q; G  8  Detecting Mycoplasma Contamination in Cell Cultures  
: `" c: u2 Q! J& v* o# Vby Polymerase Chain Reaction .......................................  93
# a3 I/ v0 @' Q2 r& x' N, iCord C. Uphoff and Hans G. Drexler % O( X& S3 p. W8 o9 W6 N" e7 ~0 S3 l
  9  Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics ........  105
6 h. J- n5 o% q& S* G. N7 HCord C. Uphoff and Hans G. Drexler 3 B6 i2 y3 G% M" @" s% g; k
10  Quality Assurance and Good Laboratory Practice .........................  1159 W3 D+ m# f1 x$ [  `7 r
Louise A. Knight and Ian A. Cree . d% n8 B4 l. J0 G
11  Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning ....  125
4 K; k' r3 f6 }# wLaura Breen, Joanne Keenan, and Martin Clynes , I9 F2 `( M4 _/ [  {( n
12  Isolation and Culture of Colon Cancer Cells and Cell Lines .................  135  h+ ?  N; I  z1 J  F
Sharon Glaysher and Ian A. Cree
+ X0 F6 C" A4 `2 a! ^2 y4 }13  Isolation and Culture of Melanoma and Naevus Cells and Cell Lines...........  141/ j8 _0 r# E. z8 U
Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett
5 e: c4 C# `' F  V& O14  Isolation and Culture of Squamous Cell Carcinoma Lines ...................  151
& M+ v, [$ ]" I* k- n6 \' VKarin J. Purdie, Celine Pourreyron, and Andrew P. South
0 |: A' @! Z) x5 O4 W' L15  Isolation and Culture of Ovarian Cancer Cells and Cell Lines ................  161
3 x1 z) v# e" Q8 {. }4 |. nChristian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween,  7 m! D1 [  p' ^" A3 L  a4 n( Q5 e; m
Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz % ?% R. G3 X' Y* |. x3 g" n
16  Establishment and Culture of Leukemia–Lymphoma Cell Lines ..............  181. }0 D0 [* z# B
Hans G. Drexler - D+ W4 X5 {4 c, j
17  Isolation of Inflammatory Cells from Human Tumours.....................  201! W5 x2 O, i/ i
Marta E. Polak
3 S& r, ^5 M8 M/ ~; w0 F18  Isolation of Endothelial Cells from Human Tumors .......................  209
: Q: E* ^, G1 @! N7 t. _Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau,  
# R0 k, f1 C' G9 [/ [/ V, d0 KRoland S. Croner, and Michael Stürzl
, w% A# M+ t! \1 M' H19  Cellular Chemosensitivity Assays: An Overview ...........................  219+ I. p- I3 c) O9 Z
Venil N. Sumantran
& d* y9 F) \/ q9 r" Z9 Z20  Cell Sensitivity Assays: The MTT Assay .................................  237
9 N9 {2 [- O$ l6 v2 eJohan van Meerloo, Gertjan J.L. Kaspers, and Jacqueline Cloos
- @; B5 G9 [2 h4 ~/ Y21  Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay .........  247
( m6 I$ N+ V4 X/ c( XSharon Glaysher and Ian A. Cree
+ O0 M# R3 d2 C" C# t/ B" k22  Differential Staining Cytotoxicity Assay: A Review ........................  259
. u, I' b: h$ O. \& y3 jLarry M. Weisenthal - b: c; t% V' v
23  Real-Time Cytotoxicity Assays .......................................  285
8 s6 i8 x( D/ ?0 X, y. u$ UDonald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz,  , L  [# n  r" m4 Q; `6 U
and Jonathan M. Cooper
) }( F0 b+ {" e8 [- e24  Purification of Annexin V and Its Use in the Detection of Apoptotic Cells ......  293
) S- x$ \" K  VKaty M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss
- q! a$ ]' @& ?  y4 d25  Measurement of DNA Damage in Individual Cells Using the Single Cell Gel 4 R! _, O* L" {4 R
Electrophoresis (Comet) Assay .......................................  309
" U' Y3 Y. e! x; d; ZJanet M. Hartley, Victoria J. Spanswick, and John A. Hartley
# ~8 ?3 V2 `( k3 K% y: O26  Molecular Breakpoint Analysis of Chromosome Translocations  
+ \2 w3 K1 ]- X; K- _in Cancer Cell Lines by Long Distance Inverse-PCR .......................  321
2 `! G2 y+ y3 EBjörn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod * H0 a' Y: @( S6 O3 G; Q) r
27  Cell Migration and Invasion Assays ....................................  333
/ O" g( p) X. O7 b# I7 G: `Karwan A. Moutasim, Maria L. Nystrom, and Gareth J. Thomas 9 H- _, N  G7 g
28  Angiogenesis Assays ...............................................  345. X1 z# c- m/ E3 A1 f7 x
V. Poulaki 2 J( U' b5 z% i; x: K9 u
29  Flow Cytometric DNA Analysis of Human Cancers and Cell Lines ............  359& ^1 P# r6 f, I& |1 v1 t7 b& j' B
Sarah A. Krueger and George D. Wilson
/ f& Q' K1 \6 f  |4 S- e30  Expression Analysis of Homeobox Genes in Leukemia/
$ i' e$ Z: m. X' F+ x0 xLymphoma Cell Lines .............................................  371
" ]5 q7 C0 {7 _0 J- S. ?Stefan Nagel and Hans G. Drexler
0 Q2 W0 f% U8 ], }. M8 |31  Measuring Gene Expression from Cell Cultures by Quantitative  " W* b$ V. G( \( D( O! O9 q& G  v
Reverse-Transcriptase Polymerase Chain Reaction.........................  3816 S8 K. k6 Y0 G+ w
Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree % S! B' U2 a6 X* B
32  Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins ....  395
9 z& A. p7 O" Q% C+ P/ ISamantha Larkin and Claire Aukim-Hastie
! D- J; Q1 f& h. R& w33  Development of Rituximab-Resistant B-NHL Clones: An In Vitro  , }  J" p+ _) ]
Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated
% {" i) X+ n8 Q8 b7 r' s3 X% d0 QImmunotherapy ..................................................  407
8 w- n7 o6 b0 g3 G2 f4 j+ `4 c, uAli R. Jazirehi and Benjamin Bonavida 1 |& s* V! W' b
34  Analysis of Drug Interactions ........................................  421
( K# O7 K3 n% g( U7 zIrene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters
" _5 {5 R" O1 R35  Transfection and DNA-Mediated Gene Transfer ..........................  435
5 P; I5 V, I  b8 H' g' X' G& E- cDavide Zecchin and Federica Di Nicolantonio
  ]$ z' O' b* y2 X* d1 rixContents1 t: }1 M9 T/ o
36  Drug Design and Testing: Profiling of Antiproliferative  
8 E( E# m5 G1 {$ v" B4 m$ [Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]- + d* ?1 H8 i- }. B- L  J/ i/ }
Thymidine Incorporation Assay ......................................  451: j0 X4 p) S) F( L
Matthew Griffiths and Hardy Sundaram
* x- m/ R* h8 k6 @% Q1 t( m37  Feeder Layers: Co-culture with Nonneoplastic Cells .......................  4679 H) U( H+ X" j5 w; y7 g
Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South 3 |: ^+ U% j" m% @/ o4 x! t
38  Xenotransplantation of Breast Cancers .................................  471
+ X6 j& u: C( t$ I( U8 ZMassimiliano Cariati, Rebecca Marlow, and Gabriela Dontu
2 u  ]# S: C+ s" c3 N, w% Z' N8 TAppendix A .........................................................  483
% |* z+ y/ I) VAppendix B .........................................................  487, i* t$ y3 F. T6 W
Index ..............................................................  493

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